Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method
	Ji Shiqi 1; Zhao Rui 1; Yin Qi 1; Zhao Yuan 2; Liu Chenguang 1; Xiao Tian 2; Zhang Xiaohua 1
刊名	JOURNAL OF OCEAN UNIVERSITY OF CHINA
	2012-03-01
卷号	11 期号:1 页码:45-51
关键词	GMD cultivation marine microorganism bacterial cultivability amphioxus
ISSN号	1672-5182
通讯作者	Zhang, XH (reprint author), Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China.
英文摘要	A gel microbead (GMD) cultivation method was employed to cultivate microorganisms from an amphioxus breeding zone in Qingdao, P. R. China. The culture results were compared with those by standard plating method. In the GMD-based method, the microcolony-forming GMDs were sorted by fluorescence-activated cell sorting (FACS). To further get pure cultures, a subsequent enrichment culture and a streaking purification procedure were conducted on marine R2A medium. Eighty bacterial strains isolated by the GMD-based method were randomly selected for sequencing. These isolates belonged to Alphaproteobacteria (33%), Gammaproteobacteria (44%), Bacteroidetes (11%), Actinobacteria (5%), Firmicutes (5%), Epsilonproteobacteria (1%), and Verrucomicrobia (1%), the last two groups being usually difficult to culture. The 16S rRNA gene sequences revealed a diverse community with 91.1%-100% of the bacterial rRNAs similarities. Thirteen strains were sharing 16S rRNA gene sequence which was less than 97% similar to any other rRNA genes currently deposited in TYP16S database. Seventy isolates derived from the standard plating method fell into 4 different taxonomic groups: Alphaproteobacteria (9%), Gammaproteobacteria (81%), Bacteroidetes (7%) and Firmicutes (3%) with a 16S rRNA gene sequence similarities between 95.8%-100%, in which only 3 strains were sharing 16S rRNA gene sequence of less than 97%. The results indicated that the GMD-based method with subenrichment culture yielded more taxonomic groups and more novel microbial strains, including members of previously rarely cultured groups, when compared with the standard plating method, and that this method markedly improved the bacterial cultivability.
学科主题	Oceanography
收录类别	SCI
原文出处	10.1007/s11802-012-1869-y
语种	英语
WOS记录号	WOS:000299527100007
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12430]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Ji Shiqi,Zhao Rui,Yin Qi,et al. Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2012,11(1):45-51.
APA	Ji Shiqi.,Zhao Rui.,Yin Qi.,Zhao Yuan.,Liu Chenguang.,...&Zhang Xiaohua.(2012).Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method.JOURNAL OF OCEAN UNIVERSITY OF CHINA,11(1),45-51.
MLA	Ji Shiqi,et al."Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method".JOURNAL OF OCEAN UNIVERSITY OF CHINA 11.1(2012):45-51.