Dual-tautomerism separation method based on asymmetric transformation: Gram-scale preparation of high-purity punicalagin from pomegranate peel wastes | |
Sun, GY (Sun, Guangying) 1; Abuduaini, M (Abuduaini, Munire) 1; Adili, G (Adili, Guliqire) 1; Zhao, YX (Zhao, Yongxin) 1; Aisa, HA (Aisa, Haji Akber) 1 | |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
2021 | |
卷号 | 1651期号:8页码:1-9 |
关键词 | Dual-tautomerism separationAsymmetric transformationLow-temperature injectionGram scalePunicalaginPomegranate peel waste |
ISSN号 | 0021-9673 |
DOI | 10.1016/j.chroma.2021.462281 |
英文摘要 | In this study, a special orthogonal separation method, named as dual-tautomerism separation (DTS), was developed for the purification of tautomeric compounds from complex matrixes. In DTS, isomers of these compounds are individually collected and asymmetrically transformed to the mixtures of isomers. After separating the mixture with an identical method, high-purity compounds can be prepared from the newly generated isomers but impurities remain in another one. To validate the effectiveness, a DTS was developed to prepare punicalagin in gram-scale from pomegranate peel waste. Isomerization kinetic and thermodynamic of punicalagin were accurately assayed by dynamic HPLC built on low-temperature or/and loop-based stop-flow two-dimensional liquid chromatography. After the isomerization based on it, 9.3 g of pomegranate peel extract was firstly separated on C18 column, and F-alpha and F-beta around alpha-punicalagin and beta-punicalagin were obtained. Then, the proportion of alpha-punicalagin in F-alpha and F-beta was optimized to 52.7% and 32.0% based on isomerization kinetics and thermodynamic. With the aid of low-temperature injection, F-alpha and F-beta were loaded and secondly purified. After waste recycling, totally 3.0 g of punicalagin with the purify of 99.5% was obtained within two days, which would strongly support the resource utilization of pomegranate peel waste. Because only an individual method was employed in the two-step purification, the separation in DTS was fully compatible. |
WOS记录号 | WOS:000663585000002 |
内容类型 | 期刊论文 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/7850] |
专题 | 新疆理化技术研究所_资源化学研究室 |
通讯作者 | Aisa, HA (Aisa, Haji Akber) 1 |
作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Xinjiang, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, GY ,Abuduaini, M ,Adili, G ,et al. Dual-tautomerism separation method based on asymmetric transformation: Gram-scale preparation of high-purity punicalagin from pomegranate peel wastes[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1651(8):1-9. |
APA | Sun, GY ,Abuduaini, M ,Adili, G ,Zhao, YX ,&Aisa, HA .(2021).Dual-tautomerism separation method based on asymmetric transformation: Gram-scale preparation of high-purity punicalagin from pomegranate peel wastes.JOURNAL OF CHROMATOGRAPHY A,1651(8),1-9. |
MLA | Sun, GY ,et al."Dual-tautomerism separation method based on asymmetric transformation: Gram-scale preparation of high-purity punicalagin from pomegranate peel wastes".JOURNAL OF CHROMATOGRAPHY A 1651.8(2021):1-9. |
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