Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis

	Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis
	Li, Hongjun; Liu, Xiao; Zhang, Guofan
刊名	PLOS ONE
	2012-10-16
卷号	7 期号:10 页码:e46926
关键词	OYSTER CRASSOSTREA-GIGAS QUANTITATIVE TRAIT LOCI CHLAMYS-FARRERI JONES SALMO-SALAR REVEALS SHELL-COLOR ZHIKONG SCALLOP EASTERN OYSTER MOLLUSCAN AQUACULTURE EUCALYPTUS-GRANDIS LATES-CALCARIFER
ISSN号	1932-6203
通讯作者	Liu, X (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
英文摘要	Bay scallop (Argopecten irradians) is one of the most economically important aquaculture species in China. In this study, we constructed a consensus microsatellite-based genetic linkage map with a mapping panel containing two hybrid backcross-like families involving two subspecies of bay scallop, A. i. irradians and A. i. concentricus. One hundred sixty-one microsatellite and one phenotypic (shell color) markers were mapped to 16 linkage groups (LGs), which corresponds to the haploid chromosome number of bay scallop. The sex-specific map was 779.2 cM and 781.6 cM long in female and male, respectively, whereas the sex-averaged map spanned 849.3 cM. The average resolution of integrated map was 5.9 cM/locus and the estimated coverage was 81.3%. The proportion of distorted markers occurred more in the hybrid parents, suggesting that the segregation distortion was possibly resulted from heterospecific interaction between genomes of two subspecies of bay scallop. The overall female-to-male recombination rate was 1.13:1 across all linked markers in common to both parents, and considerable differences in recombination also existed among different parents in both families. Four size-related traits, including shell length (SL), shell height (SH), shell width (SW) and total weight (TW) were measured for quantitative trait loci (QTL) analysis. Three significant and six suggestive QTL were detected on five LGs. Among the three significant QTL, two (qSW-10 and qTW-10, controlling SW and TW, respectively) were mapped on the same region near marker AiAD121 on LG10 and explained 20.5% and 27.7% of the phenotypic variance, while the third (qSH-7, controlling SH) was located on LG7 and accounted for 15.8% of the phenotypic variance. Six suggestive QTL were detected on four different LGs. The linkage map and size-related QTL obtained in this study may facilitate marker-assisted selection (MAS) in bay scallop.
学科主题	Multidisciplinary Sciences
收录类别	SCI
原文出处	10.1371/journal.pone.0046926
语种	英语
WOS记录号	WOS:000310135800017
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12207]
专题	海洋研究所_海洋生物技术研发中心
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongjun,Liu, Xiao,Zhang, Guofan. Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis[J]. PLOS ONE,2012,7(10):e46926.
APA	Li, Hongjun,Liu, Xiao,&Zhang, Guofan.(2012).Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis.PLOS ONE,7(10),e46926.
MLA	Li, Hongjun,et al."Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis".PLOS ONE 7.10(2012):e46926.