Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta)

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	Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta)
	Xuan Jinsong 1,4; Feng Yanbin 1; Weng Manli 1; Zhao Ge 1; Shi Jinfeng 1; Yao Jianting 2; Wang Xiuliang 2; Guo Baotai 3; Qiao Lixian 3; Duan Delin 2
刊名	ACTA OCEANOLOGICA SINICA
	2012-11-01
卷号	31 期号:6 页码:139-148
关键词	cDNA library comparative study expressed sequence tag (EST) analysis Laminaria japonica trehalose-6-phosphate synthase (TPS) TPS gene
ISSN号	0253-505X
通讯作者	Wang, B (reprint author), Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China.
英文摘要	A high quality cDNA library was constructed from the brown alga Laminaria japonica, with the titer of 1.2x10(5) pfu/ml. The average insert size of the cDNA library is about 1.6 kb. From the cDNA library, 591 cDNA clones were randomly selected and sequenced. As a result, 574 EST (expressed sequence tag) sequences were generated. All of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942625 to CX943198. The cDNA library was screened with a alpha-(32)p labeled 453 bp TPS gene probe, which is a partial sequence yielded from Porphyra yezoensis. Four positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of L. japonica TPS cDNA sequence. After PCR amplification, sequencing and assembling, the entire ORF (open reading frame) sequence of the TPS gene was obtained, which was named LjTPS. LjTPS encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 Daltons. The LjTPS gene was successfully expressed in E. coli and rice. The LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the TPS gene function and mechanism to stress tolerance.
学科主题	Oceanography
收录类别	SCI
原文出处	10.1007/s13131-012-0260-6
语种	英语
WOS记录号	WOS:000312494000014
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12326]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, State Key Lab Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 3.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 4.Univ Sci & Technol Beijing, Dept Biol Sci & Engn, Sch Chem & Biol Engn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Xuan Jinsong,Feng Yanbin,Weng Manli,et al. Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta)[J]. ACTA OCEANOLOGICA SINICA,2012,31(6):139-148.
APA	Xuan Jinsong.,Feng Yanbin.,Weng Manli.,Zhao Ge.,Shi Jinfeng.,...&Wang Bin.(2012).Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta).ACTA OCEANOLOGICA SINICA,31(6),139-148.
MLA	Xuan Jinsong,et al."Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta)".ACTA OCEANOLOGICA SINICA 31.6(2012):139-148.