Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus

	Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus
	Song, Chengwen2,3 ; Cui, Zhaoxia 1; Liu, Yuan 2,3; Li, Qianqian2,3 ; Wang, Shuangyan 2,3
刊名	MOLECULAR BIOLOGY REPORTS
	2012-04-01
卷号	39 期号:4 页码:4879-4888
关键词	Portunus trituberculatus Arginine kinase SNPs Expression
ISSN号	0301-4851
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Arginine kinase (AK) is an important phosphotransferase that plays a critical role in energy metabolism in invertebrates. In this paper, the cDNA of AK (designated as PtAK) was identified from the eyestalk cDNA library of swimming crab Portunus trituberculatus. The full-length cDNA was 1,479 bp, containing an open reading frame of 1,074 bp that coded for 357 amino acids. The estimated molecular mass of mature PtAK was 40.30 kDa and theoretical isoelectric point was 6.18. Amino acid sequence alignment showed that PtAK had very high similarity with other shrimp and crab AKs ranging from 0.876 to 0.983. The genomic DNA fragments of about 1,434 bp consisted of two exons interrupted by an intron. Totally 24 SNPs, including 17 in the coding region and seven in the non-coding region, were detected by direct sequencing of 19 genomic samples. In exon 1, the coding SNPs (cSNPs) were only found in the disease-resistant specimens. The fluorescent real-time PCR analysis revealed that the expression of PtAK was detected in all the examined tissues with the highest expression in the muscle and the lowest in the eyestalk. The expression of PtAK after Vibrio alginolyticus injection was tested in haemocytes, showing that two peak values were 5.01-fold (at 3 h) and 3.60-fold (at 24 h) compared with the control values, respectively. The results suggested that AK might play an important role in the immune response in crabs.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
原文出处	10.1007/s11033-011-1283-3
语种	英语
WOS记录号	WOS:000301108500173
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12300]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Song, Chengwen,Cui, Zhaoxia,Liu, Yuan,et al. Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus[J]. MOLECULAR BIOLOGY REPORTS,2012,39(4):4879-4888.
APA	Song, Chengwen,Cui, Zhaoxia,Liu, Yuan,Li, Qianqian,&Wang, Shuangyan.(2012).Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus.MOLECULAR BIOLOGY REPORTS,39(4),4879-4888.
MLA	Song, Chengwen,et al."Cloning and expression of arginine kinase from a swimming crab, Portunus trituberculatus".MOLECULAR BIOLOGY REPORTS 39.4(2012):4879-4888.