Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus
	Wang, Hongxia 1; Cui, Zhaoxia 1; Wu, Danhua 1,2; Guo, Enmian 1,3; Liu, Yuan 1,4; Wang, Chunlin 2; Su, Xiurong 2; Li, Taiwu 2
刊名	AQUACULTURE INTERNATIONAL
	2012-08-01
卷号	20 期号:4 页码:649-656
关键词	Portunus trituberculatus Microsatellite DNA parentage analyses Selective breeding
ISSN号	0967-6120
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Microsatellites have been a powerful genetic tool in determining pedigree relationships among individuals. A microsatellite marker suite can be used to reliably assign parentage in the Portunus trituberculatus selective breeding programs. Thirty published microsatellites were screened for their suitability to be included in a DNA parentage marker suite. Nineteen microsatellite markers that exhibited significant stutter, poor allele resolution, null allele, and significant deviation from Mendelian expectations were eliminated from the marker suite, and the left eleven markers were ranked by PIC. Six most polymorphic markers with mean He = 0.928 and PIC = 0.901 from the original eleven were further assessed for the efficiency of pedigree identification. Total predicted power of the markers to exclude false parents was higher than 99%. Both simulations and real data analysis confirmed the high statistical power of six microsatellites suite in parentage exclusion, which permit us to trace back all offspring to single pairs of parents.
学科主题	Fisheries
收录类别	SCI
原文出处	10.1007/s10499-011-9493-1
语种	英语
WOS记录号	WOS:000306283000004
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12285]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 3.Qingdao Agr Univ, Qingdao 266109, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hongxia,Cui, Zhaoxia,Wu, Danhua,et al. Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus[J]. AQUACULTURE INTERNATIONAL,2012,20(4):649-656.
APA	Wang, Hongxia.,Cui, Zhaoxia.,Wu, Danhua.,Guo, Enmian.,Liu, Yuan.,...&Li, Taiwu.(2012).Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus.AQUACULTURE INTERNATIONAL,20(4),649-656.
MLA	Wang, Hongxia,et al."Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus".AQUACULTURE INTERNATIONAL 20.4(2012):649-656.