Inv1: An Edwardsiella tarda invasin and a protective immunogen that is required for host infection

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	Inv1: An Edwardsiella tarda invasin and a protective immunogen that is required for host infection
	Li, Mo-fei1,4 ; Hu, Yong-hua 1; Zheng, Wen-jiang 1,2,3; Sun, Bo-guang1 ; Wang, Chun-lin 4; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-04-01
卷号	32 期号:4 页码:586-592
关键词	Invasin Edwardsiella tarda Virulence Vaccine
ISSN号	1050-4648
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Invasin is an outer membrane protein that is known to mediate entry of enteric bacteria into mammalian cells. In this study, we analyzed the function and immunoprotective potential of the invasin Inv1 from Edwardsiella tarda, a serious fish pathogen that can also infect humans. In silico analysis indicated that Inv1 possesses a conserved N-terminal DUF3442 domain and a C-terminal group 1 bacterial Ig-like domain. Subcellular localization analysis showed that Inv1 is exposed on cell surface and could be recognized by specific antibodies. Mutation of inv1 had no effect on bacterial growth but attenuates overall bacterial virulence and impaired the ability of E. tarda to attach and invade into host cells. Consistent with these observations, antibody blocking of Inv1 inhibited E. tarda infection of host cells. To examine the immunoprotective potential of Inv1, recombinant Inv1 (rInv1) corresponding to the DUF3442 domain was purified and used to vaccinate Japanese flounder (Paralichthys olivaceus). The results showed that rInv1 induced strong protection against lethal-dose challenge of E. tarda. ELISA analysis showed that rInv1-vaccinated fish produced specific serum antibodies that could enhance the serum bactericidal activity against E. tarda. Taken together, these results indicate that Inv1 is a surface-localized virulence factor that is involved in host infection and can induce effective immunoprotection when used as a subunit vaccine. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.01.016
语种	英语
WOS记录号	WOS:000301827100010
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12279]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Natl Sci Lib, Lanzhou Branch, Lanzhou, Gansu, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Li, Mo-fei,Hu, Yong-hua,Zheng, Wen-jiang,et al. Inv1: An Edwardsiella tarda invasin and a protective immunogen that is required for host infection[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):586-592.
APA	Li, Mo-fei,Hu, Yong-hua,Zheng, Wen-jiang,Sun, Bo-guang,Wang, Chun-lin,&Sun, Li.(2012).Inv1: An Edwardsiella tarda invasin and a protective immunogen that is required for host infection.FISH & SHELLFISH IMMUNOLOGY,32(4),586-592.
MLA	Li, Mo-fei,et al."Inv1: An Edwardsiella tarda invasin and a protective immunogen that is required for host infection".FISH & SHELLFISH IMMUNOLOGY 32.4(2012):586-592.