MALDI-MS imaging of lipids and small molecules in rat brain tissue based on graphene oxide film pre-coated matrix

doi:10.1016/j.ijms.2021.116573

	MALDI-MS imaging of lipids and small molecules in rat brain tissue based on graphene oxide film pre-coated matrix
	Xu, Yang 1; Deng, Yingzhi 2; Ye, Rongrong 1; Gong, Can 1; Liu, Zhaoxin 1; Zhao, Yuezhen 1; Lu, Yan 1; Liu, Jinggen 2; Xu, Xu 1
刊名	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
	2021-06-01
卷号	464 页码:8
关键词	Single-layer graphene oxide film Matrix-assisted laser desorption ionization-mass spectrometry imaging Phospholipids Pre-coated matrix
ISSN号	1387-3806
DOI	10.1016/j.ijms.2021.116573
通讯作者	Lu, Yan(yanluu@sit.edu.cn) ; Xu, Xu(xuxu@sit.edu.cn)
英文摘要	In the study, single-layer graphene oxide (SLGO) was adsorbed on a conductive glass surface to prepare a uniform thin film. We used this film as the pre-coated matrix in matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging to avoid the need of a matrix cover. We investigated the effects of the uniformity and matrix amount required to prepare the pre-coated matrix film for the MALDI-MS experiment. The SLGO water-dispersed suspension was centrifuged to remove the suspended particles and improve the film uniformity. We adopted the SLGO (0.75 mg) and a laser energy of 65%. The MALDI-MS experiment based on the pre-coated SLGO thin film presented repeatabilities (relative standard deviation) of less than 4% for four kinds of chemical samples. Through the MALDI-MS imaging based on the pre-coated matrix, we detected more than 60 kinds of lipids and small molecules, including phospholipids, cyclic adenosine monophosphate, inosine, and cholesterol, in a rat brain tissue slice. We further demonstrated the spatial distribution of the detected compounds in the rat brain tissue by imaging. The conductive glass covered with the SLGO thin film could be stored for more than one month and used directly in MALDI-MS imaging, which eliminated the need for a matrix cover and simplified the MALDI-MS imaging experimental process. (c) 2021 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[31671928] ; National Natural Science Foundation of China[21505093] ; Natural Science Foundation of Shanghai[15ZR1440800]
WOS关键词	LASER-DESORPTION IONIZATION ; DESORPTION/IONIZATION MASS-SPECTROMETRY ; BIOLOGICAL TISSUES ; ASSISTED MATRIX ; FATTY-ACIDS ; THIN-FILM ; SAMPLES ; TARGETS ; NANOPARTICLES ; PHOSPHOLIPIDS
WOS研究方向	Physics ; Spectroscopy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000640058600007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296508]
专题	中国科学院上海药物研究所
通讯作者	Lu, Yan; Xu, Xu
作者单位	1.Shanghai Inst Technol, Sch Chem & Environm Engn, 100 Haiquan Rd, Shanghai 201418, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Xu, Yang,Deng, Yingzhi,Ye, Rongrong,et al. MALDI-MS imaging of lipids and small molecules in rat brain tissue based on graphene oxide film pre-coated matrix[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2021,464:8.
APA	Xu, Yang.,Deng, Yingzhi.,Ye, Rongrong.,Gong, Can.,Liu, Zhaoxin.,...&Xu, Xu.(2021).MALDI-MS imaging of lipids and small molecules in rat brain tissue based on graphene oxide film pre-coated matrix.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,464,8.
MLA	Xu, Yang,et al."MALDI-MS imaging of lipids and small molecules in rat brain tissue based on graphene oxide film pre-coated matrix".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 464(2021):8.