A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography

doi:10.1007/s00418-020-01949-8

	A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography
	Jiang, Liyuan 2,4,5,6; Cao, Yong 2,4,5,6; Yin, Xianzhen 1; Ni, Shuangfei 2,4,5,6; Li, Miao 2,4,5,6; Li, Chengjun 2,4,5,6; Luo, Zixiang 2,4,5,6; Lu, Hongbin 2,3,4,5; Hu, Jianzhong 2,4,5,6
刊名	HISTOCHEMISTRY AND CELL BIOLOGY
	2021-01-04
页码	13
关键词	SRμ CT Modified Golgi-Cox method Spinal cord Neuronal network Three-dimension
ISSN号	0948-6143
DOI	10.1007/s00418-020-01949-8
通讯作者	Lu, Hongbin(hongbinlu@hotmail.com) ; Hu, Jianzhong(Jianzhonghu@hotmail.com)
英文摘要	Exploring the three-dimensional (3D) morphology of neurons is essential to understanding spinal cord function and associated diseases comprehensively. However, 3D imaging of the neuronal network in the broad region of the spinal cord at cellular resolution remains a challenge in the field of neuroscience. In this study, to obtain high-resolution 3D imaging of a detailed neuronal network in the mass of the spinal cord, the combination of synchrotron radiation micro-computed tomography (SR mu CT) and the Golgi-cox staining were used. We optimized the Golgi-Cox method (GCM) and developed a modified GCM (M-GCM), which improved background staining, reduced the number of artefacts, and diminished the impact of incomplete vasculature compared to the current GCM. Moreover, we achieved high-resolution 3D imaging of the detailed neuronal network in the spinal cord through the combination of SR mu CT and M-GCM. Our results showed that the M-GCM increased the contrast between the neuronal structure and its surrounding extracellular matrix. Compared to the GCM, the M-GCM also diminished the impact of the artefacts and incomplete vasculature on the 3D image. Additionally, the 3D neuronal architecture was successfully quantified using a combination of SR mu CT and M-GCM. The SR mu CT was shown to be a valuable non-destructive tool for 3D visualization of the neuronal network in the broad 3D region of the spinal cord. Such a combinatorial method will, therefore, transform the presentation of Golgi staining from 2 to 3D, providing significant improvements in the 3D rendering of the neuronal network.
资助项目	National Natural Science Foundation of China[81371956 81874004] ; National Natural Science Foundation of China[81672174] ; National Natural Science Foundation of China[81730068] ; Hunan Provincial Department of Finance[(2018)82] ; Fundamental Research Funds for the Central Universities of Central South University[2018zzts254] ; Key R&D Program of the Hunan Provincial Science & Technology Department[2017SK2061]
WOS关键词	X-RAY MICROTOMOGRAPHY ; SYSTEM ; TISSUE ; HISTOLOGY ; SAMPLES ; MODEL
WOS研究方向	Cell Biology ; Microscopy
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000604876700005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296054]
专题	中国科学院上海药物研究所
通讯作者	Lu, Hongbin; Hu, Jianzhong
作者单位	1.Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Key Lab Organ Injury Aging & Regenerat Med Hunan, Changsha 410008, Peoples R China 3.Cent South Univ, Xiangya Hosp, Dept Sports Med, Xiangya Rd 87, Changsha 410008, Hunan, Peoples R China 4.Xiangya Hosp, Int Chinese Musculoskeletal Res Soc, Sports Med Res Ctr, Changsha, Peoples R China 5.Hunan Engn Res Ctr Sport & Hlth, Changsha, Peoples R China 6.Cent South Univ, Xiangya Hosp, Dept Spine Surg, Xiangya Rd 87, Changsha 410008, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Liyuan,Cao, Yong,Yin, Xianzhen,et al. A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2021:13.
APA	Jiang, Liyuan.,Cao, Yong.,Yin, Xianzhen.,Ni, Shuangfei.,Li, Miao.,...&Hu, Jianzhong.(2021).A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography.HISTOCHEMISTRY AND CELL BIOLOGY,13.
MLA	Jiang, Liyuan,et al."A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography".HISTOCHEMISTRY AND CELL BIOLOGY (2021):13.