Chronic administration of synthetic cannabidiol induces antidepressant effects involving modulation of serotonin and noradrenaline levels in the hippocampus

doi:10.1016/j.neulet.2020.135594

	Chronic administration of synthetic cannabidiol induces antidepressant effects involving modulation of serotonin and noradrenaline levels in the hippocampus
	Abame, Melkamu Alemu 1,2; He, Yang 1; Wu, Song 3; Xie, Zhifei 3; Zhang, Jian 3; Gong, Xudong 3; Wu, Chunhui 3; Shen, Jingshan 1,2
刊名	NEUROSCIENCE LETTERS
	2021-01-23
卷号	744 页码:7
关键词	Synthetic cannabidiol Depression Serotonin Noradrenaline Nuclear factor kappa B
ISSN号	0304-3940
DOI	10.1016/j.neulet.2020.135594
通讯作者	Wu, Chunhui() ; Shen, Jingshan(shenjingshan@simm.ac.cn)
英文摘要	Cannabidiol (CBD) is a non-psychotomimetic compound derived from Cannabis sativa. Preclinical and clinical studies have shown therapeutic potential of CBD in a variety of disorders. Despite several research efforts on CBD, its antidepressant activity has been poorly investigated and the exact mechanism of action remains unclear. Thus, this study aimed to further explore the mechanism of CBD after chronic administration (7 days). First, the dose level of CBD that is enough to produce antidepressant effects after chronic administration was explored. Second, the changes in key proteins and neurotransmitters through such methods as real-time polymerase chain reaction (RT-PCR), western blotting, and high-performance liquid chromatography-electrochemical detection (HPLC-ECD) were critically studied. Furthermore, correlation between behavioral phenotypes with protein and neurotransmitters was established and the possible mechanism was herein postulated. The results showed that only the high dose CBD 100 mg/kg chronic administration induced antidepressant-like effects in mice subjected to forced swim test. Chronic CBD 100 mg/kg administration resulted in significant increases in serotonin (5-HT) and noradrenaline (NA) levels in the hippocampus (HPC). Similarly, the chronic administration of CBD 30 mg/kg and CBD 100 mg/kg significantly decreased nuclear factor kappa B (NF-kappa B) expression in the HPC. Moreover, none of the treatments were observed to induce locomotor effects. Thus, we concluded that chronic administration of CBD (100 mg/kg) induced antidepressant-like effects by increasing 5-HT and NA levels in the HPC. These results shed new light on further discovery of the antidepressant effect of CBD.
资助项目	Special Foundation of Chinese Academy of Sciences for strategic pilot technology[XDA12040337] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002] ; Belt and Road master fellowship
WOS关键词	INVOLVEMENT ; DEPRESSION
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000608847300011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/295949]
专题	中国科学院上海药物研究所
通讯作者	Wu, Chunhui; Shen, Jingshan
作者单位	1.Chinese Acad Sci, Drug Discovery & Design Ctr, Shanghai Inst Mat Medial, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Sch Pharm, Beijing 100049, Peoples R China 3.Topharman Shanghai Co Ltd, Dept Druggabilty Evaluat, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Abame, Melkamu Alemu,He, Yang,Wu, Song,et al. Chronic administration of synthetic cannabidiol induces antidepressant effects involving modulation of serotonin and noradrenaline levels in the hippocampus[J]. NEUROSCIENCE LETTERS,2021,744:7.
APA	Abame, Melkamu Alemu.,He, Yang.,Wu, Song.,Xie, Zhifei.,Zhang, Jian.,...&Shen, Jingshan.(2021).Chronic administration of synthetic cannabidiol induces antidepressant effects involving modulation of serotonin and noradrenaline levels in the hippocampus.NEUROSCIENCE LETTERS,744,7.
MLA	Abame, Melkamu Alemu,et al."Chronic administration of synthetic cannabidiol induces antidepressant effects involving modulation of serotonin and noradrenaline levels in the hippocampus".NEUROSCIENCE LETTERS 744(2021):7.