Engineering Nanoscale Artificial Antigen-Presenting Cells by Metabolic Dendritic Cell Labeling to Potentiate Cancer Immunotherapy

doi:10.1021/acs.nanolett.0c04783

	Engineering Nanoscale Artificial Antigen-Presenting Cells by Metabolic Dendritic Cell Labeling to Potentiate Cancer Immunotherapy
	Xiao, Ping 1,2; Wang, Jue 1,2; Zhao, Zitong 2; Liu, Xiaochen 1,2; Sun, Xiangshi 2; Wang, Dangge 2,3,4; Li, Yaping 1,2,4
刊名	NANO LETTERS
	2021-03-10
卷号	21 期号:5 页码:2094-2103
关键词	nanoscale artificial antigen-presenting cells metabolic cell labeling dendritic cells cancer immunotherapy
ISSN号	1530-6984
DOI	10.1021/acs.nanolett.0c04783
通讯作者	Wang, Dangge() ; Li, Yaping(ypli@simm.ac.cn)
英文摘要	Nanoscale artificial antigen-presenting cells (aAPCs) are promising to activate T cells directly for cancer immunotherapy, while feasible and flexible strategy to develop nanoscale aAPCs remains highly desirable. Metabolic glycoengineering is used to decorate chemical tags on cells which enables bioorthogonal chemical conjugation of functional molecules. Herein, we develop a nanoscale aAPC by metabolic dendritic cell (DC) labeling to mobilize T-cell based antitumor immunity. We coat azido-labeled DC membrane on imiquimod-loaded polymeric nanoparticles and sequentially modify anti-CD3 epsilon antibody via click chemistry. The nanoscale aAPCs perform improved distribution in lymph nodes and stimulate T cells and resident APCs. Significant inhibition of tumor inoculation and growth is observed after the vaccination, which can be further improved by combining antiprogrammed cell death receptor 1 (PD1) therapy. Our results demonstrate the promising application of metabolically labeled DCs for designing nanoscale aAPCs, which provide a simple and general strategy to potentiate cancer immunotherapy.
资助项目	National Natural Science Foundation of China[81903548] ; National Natural Science Foundation of China[81690265] ; National Natural Science Foundation of China[81803444] ; National Natural Science Foundation of China[81521005] ; National Natural Science Foundation of China[32070927] ; Strategic Priority Research Program of CAS[XDA12050307] ; Youth Innovation Promotion Association of CAS[2019283] ; International Partnership Program of CAS[153631KYSB20190013] ; Shanghai Sailing Program[19YF1457300]
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000629091100027
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/295382]
专题	中国科学院上海药物研究所
通讯作者	Wang, Dangge; Li, Yaping
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, State Key Lab Drug Res & Ctr Pharmaceut, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Yantai Univ, Sch Pharm, Key Lab Mol Pharmacol & Drug Evaluat, Minist Educ, Yantai 264005, Peoples R China 4.Yantai Inst Mat Med, Yantai Key Lab Nanomed & Adv Preparat, Yantai 265700, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Ping,Wang, Jue,Zhao, Zitong,et al. Engineering Nanoscale Artificial Antigen-Presenting Cells by Metabolic Dendritic Cell Labeling to Potentiate Cancer Immunotherapy[J]. NANO LETTERS,2021,21(5):2094-2103.
APA	Xiao, Ping.,Wang, Jue.,Zhao, Zitong.,Liu, Xiaochen.,Sun, Xiangshi.,...&Li, Yaping.(2021).Engineering Nanoscale Artificial Antigen-Presenting Cells by Metabolic Dendritic Cell Labeling to Potentiate Cancer Immunotherapy.NANO LETTERS,21(5),2094-2103.
MLA	Xiao, Ping,et al."Engineering Nanoscale Artificial Antigen-Presenting Cells by Metabolic Dendritic Cell Labeling to Potentiate Cancer Immunotherapy".NANO LETTERS 21.5(2021):2094-2103.