Exploration of tissue distribution of ginsenoside Rg1 by LC-MS/MS and nanospray desorption electrospray ionization mass spectrometry

doi:10.1016/j.jpba.2021.113999

	Exploration of tissue distribution of ginsenoside Rg1 by LC-MS/MS and nanospray desorption electrospray ionization mass spectrometry
	Wei, Wenlong 1; Li, Zhenwei 1,2; Li, Haojv 1,2; An, Yaling 1,2; Qu, Hua 1; Yao, Changliang 1; Zhang, Jianqing 1; Li, Jiayuan 1; Zhang, Gaole 1; Shi, Yanchao 3
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2021-05-10
卷号	198 页码:7
关键词	Ginsenoside Rg1 Nanospray desorption electrospray ionization Tissue distribution Spatial distribution
ISSN号	0731-7085
DOI	10.1016/j.jpba.2021.113999
通讯作者	Guo, De-an(daguo@simm.ac.cn)
英文摘要	Ginsenoside Rg1 (Rg1) was one of the dominent active components in several Panax medicinal species as Panax notoginseng and Panaxginseng with diversified bioactivities. However, the study on tissue distribution of Rg1 remained limited and needed to be further explored for elucidation of its spatial distribution. In the present study, a LC-MS/MS combined with nanospray desorption electrospray ionization (DESI) mass spectrometry method was developed for exploration of tissue distribution of Rg1 at different time points after intravenous administration to rats. Furthermore, a MS inlet-heat method was developed to improve the imaging efficacy of Rg1 in brain tissue. The results obtained from LC-MS/MS analysis indicated that kidney possessed the highest tissue concentration, followed by liver, lung, spleen, heart and brain. Meanwhile, the elimination of Rg1 was swift within 1 h. For the spatial distribution of Rg1 by DESI-MS, Rg1 mainly accumulated in the pelvis section of kidney. Meanwhile, the imaging result of brain implied that Rg1 might be distributed in the pons and medulla oblongata region of brain at 15 min after intravenous administration. It is anticipated that the data on tissue distribution of Rg1 could provide references for further probing its efficacy and drug development. (C) 2021 Published by Elsevier B.V.
资助项目	National Key R&D Program of China[2019YFC1711000] ; National Natural Science Foundation of China[81530095] ; National Natural Science Foundation of China[81673591] ; Strategic Priority ResearchProgram of the Chinese Academy of Sciences[XDA12020348] ; National Standardization of Traditional Chinese Medicine Project[ZYBZH K LN 01] ; Science and Technology Commission Foundation of Shanghai[15DZ0502800] ; Projects of research and develop plan in key field of Guangdong[2020B1111110007]
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000636654000018
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/295226]
专题	中国科学院上海药物研究所
通讯作者	Guo, De-an
作者单位	1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Waters Corp Shanghai Sci & Technol Co Ltd, Bldg 13,1000 Jinhai Rd, Shanghai 200000, Peoples R China
推荐引用方式 GB/T 7714	Wei, Wenlong,Li, Zhenwei,Li, Haojv,et al. Exploration of tissue distribution of ginsenoside Rg1 by LC-MS/MS and nanospray desorption electrospray ionization mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2021,198:7.
APA	Wei, Wenlong.,Li, Zhenwei.,Li, Haojv.,An, Yaling.,Qu, Hua.,...&Guo, De-an.(2021).Exploration of tissue distribution of ginsenoside Rg1 by LC-MS/MS and nanospray desorption electrospray ionization mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,198,7.
MLA	Wei, Wenlong,et al."Exploration of tissue distribution of ginsenoside Rg1 by LC-MS/MS and nanospray desorption electrospray ionization mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 198(2021):7.