Reversal of multidrug resistance by icaritin in doxorubicin-resistant human osteosarcoma cells

	Reversal of multidrug resistance by icaritin in doxorubicin-resistant human osteosarcoma cells
	Wang ZhenDong; Wang RuiZhi; Xia YuanZheng; Kong LingYi; Yang Lei
刊名	CHINESE JOURNAL OF NATURAL MEDICINES
	2018
卷号	16 期号:1 页码:20
关键词	CHEMOTHERAPEUTIC-AGENTS HERBA-EPIMEDII CANCER-CELLS TRANSPORTERS EXPRESSION FLAVONOIDS PROTEIN-1 ABCB1 PHARMACOKINETICS PROLIFERATION Osteosarcoma Icaritin Multidrug resistance MDR1 MRP1
ISSN号	2095-6975
英文摘要	Multidrug resistance (MDR) is one of the major obstacles in cancer chemotherapy. Our previous study has shown that icariin could reverse MDR in MG-63 doxorubicin-resistant (MG-63/DOX) cells. It is reported that icariin is usually metabolized to icariside II and icaritin. Herein, we investigated the effects of icariin, icariside II, and icaritin (ICT) on reversing MDR in MG-63/DOX cells. Among these compounds, ICT exhibited strongest effect and showed no obvious cytotoxicity effect on both MG-63 and MG-63/DOX cells ranging from 1 to 10 mu mol.L-1. Furthermore, ICT increased accumulation of rhodamine 123 and 6-carboxyfluorescein diacetate and enhanced DOX-induced apoptosis in MG-63/DOX cells in a dose-dependent manner. Further studies demonstrated that ICT decreased the mRNA and protein levels of multidrug resistance protein 1 (MDR1) and multidrug resistance-associated protein 1 (MRP1). We also verified that blockade of STAT3 phosphorylation was involved in the reversal effect of multidrug resistance in MG-63/DOX cells. Taken together, these results indicated that ICT may be a potential candidate in chemotherapy for osteosarcoma.
资助项目	[National Natural Science Foundation of China] ; [Natural Science Foundation of Jiangsu Province] ; [Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)] ; [Program for Changjiang Scholars and Innovative Research Team in University]
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/294171]
专题	中国科学院上海药物研究所
作者单位	中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Wang ZhenDong,Wang RuiZhi,Xia YuanZheng,et al. Reversal of multidrug resistance by icaritin in doxorubicin-resistant human osteosarcoma cells[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2018,16(1):20.
APA	Wang ZhenDong,Wang RuiZhi,Xia YuanZheng,Kong LingYi,&Yang Lei.(2018).Reversal of multidrug resistance by icaritin in doxorubicin-resistant human osteosarcoma cells.CHINESE JOURNAL OF NATURAL MEDICINES,16(1),20.
MLA	Wang ZhenDong,et al."Reversal of multidrug resistance by icaritin in doxorubicin-resistant human osteosarcoma cells".CHINESE JOURNAL OF NATURAL MEDICINES 16.1(2018):20.