Development of fluorinated polyplex nanoemulsions for improved small interfering RNA delivery and cancer therapy

	Development of fluorinated polyplex nanoemulsions for improved small interfering RNA delivery and cancer therapy
	Chen Gang 1; Wang Kaikai 1; Wu Pengkai 1; Wang Yixin 1; Zhou Zhanwei 1; Yin Lifang 1; Sun Minjie 1; Oupicky David 1
刊名	NANO RESEARCH
	2018
卷号	11 期号:7 页码:3746
关键词	MOLECULAR-WEIGHT POLYETHYLENIMINE DRUG-DELIVERY GENE DELIVERY PERFLUOROCARBON NANOEMULSION TRANSFECTION EFFICACY IN-VITRO DNA NANOPARTICLES CARRIERS CELL polyplexes fluorous interactions small interfering RNA (siRNA) delivery tumor delivery perfluorocarbon emulsion
ISSN号	1998-0124
英文摘要	We report the development of a small interfering RNA (siRNA) delivery vector based on cationic perfluorocarbon nanoemulsions. We have prepared perfluorodecalin (PFD) emulsions with a positive surface charge provided by a fluorinated poly(ethylenimine) (F-PEI). The fluorinated emulsion (F-PEI@PFD) reduced cytotoxicity of F-PEI and demonstrated effective binding with siRNAs to form nanosized emulsion polyplexes. The prepared emulsion polyplexes enhanced cellular uptake and improved endosomal escape of the siRNA. In addition to increased reporter gene silencing in multiple cancer cell lines, when compared with control F-PEI and PEI polyplexes, the siRNA emulsion polyplexes showed an excellent resistance to serum deactivation and maintained high activity, even in high-serum conditions. The F-PEI@PFD emulsion polyplexes carrying an siRNA to silence the expression of Bcl2 gene induced apoptosis and inhibited tumor growth in a melanoma mouse model in vivo and showed potential for in vivo ultrasound imaging. This study demonstrates the potential of F-PEI@PFD emulsions as a multifunctional theranostic nanoplatform for safe siRNA delivery, with integrated ultrasound imaging functionality.
资助项目	[National Science and Technology Major Project] ; [Changjiang Scholar program] ; [National Natural Science Foundation of China] ; [China Postdoctoral Science Foundation] ; [Postgraduate Research & Practice Innovation Program of Jiangsu Province]
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/292939]
专题	中国科学院上海药物研究所
作者单位	1.中国科学院南京地理与湖泊研究所 2.中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Chen Gang,Wang Kaikai,Wu Pengkai,et al. Development of fluorinated polyplex nanoemulsions for improved small interfering RNA delivery and cancer therapy[J]. NANO RESEARCH,2018,11(7):3746.
APA	Chen Gang.,Wang Kaikai.,Wu Pengkai.,Wang Yixin.,Zhou Zhanwei.,...&Oupicky David.(2018).Development of fluorinated polyplex nanoemulsions for improved small interfering RNA delivery and cancer therapy.NANO RESEARCH,11(7),3746.
MLA	Chen Gang,et al."Development of fluorinated polyplex nanoemulsions for improved small interfering RNA delivery and cancer therapy".NANO RESEARCH 11.7(2018):3746.