A potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol-induced rat model

doi:10.1016/j.intimp.2020.106660

	A potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol-induced rat model
	Xie, Lu 7; Guo, Yi-lin 1,2; Chen, Yu-rou 7; Zhang, Li-ying 3; Wang, Zhi-cheng 6; Zhang, Tong 1,2; Wang, Bing 2,4,5
刊名	INTERNATIONAL IMMUNOPHARMACOLOGY
	2020-08-01
卷号	85 页码:9
关键词	Gastric ulcer Omeprazole Patchouli alcohol
ISSN号	1567-5769
DOI	10.1016/j.intimp.2020.106660
通讯作者	Wang, Zhi-cheng(ahwzc@126.com) ; Zhang, Tong(zhangtong@shutcm.edu.cn) ; Wang, Bing(bwang@simm.ac.cn)
英文摘要	Omeprazole (OME) is a representative of proton pump inhibitors and widely used in anti-ulcer treatment. However, OME may cause some inevitable side effects and the long-term consequences of OME could increase the risk of diarrhea. Patchouli Alcohol (PA), the main extract of Pogostemonis Herba, have demonstrated benefits in treating gastric ulcer (GU) with low toxicity. The present study aimed to investigate the synergistically protective effects of OME and PA against ethanol-induced GU in rats to study the involvement of antioxidant and anti-inflammatory activities. Moreover, the anti-apoptosis, anti-oxidant and anti-inflammatory effects in H2O2-induced gastric epithelial cells (GES-1) and LPS-induced RAW264.7 cells were determined, as well as the modulation of signaling proteins. The results demonstrated that PA alone or combined with OME provided remarkable benefits by reducing ulcer areas, modulating oxidant stress and inflammatory factors and the therapeutic efficacy was showed to be dose-dependent, which were partly superior to that of high-dose OME only. Additionally, co-treated regimen could superiorly down-regulate cell apoptosis and regulate the levels of oxidant activities and inflammatory cytokines on H2O2-induced GES-1 cells and LPS-induced RAW264.7 cells, which involved with cleaved caspase 3, Bcl-2 and BAX protein expressions and MAPK pathway. We provided a new understanding that the combination of OME and PA possessed gastroprotective effects on modulating cell apoptosis, antioxidant stress and anti-inflammatory responses against GU. Therefore, PA was inferred to take a potential and critic role in gastric mucosa protection.
资助项目	Program of Science and Technology Commission of Shanghai Municipality, China[17401902300] ; Program of Shanghai Academic/Technology Research Leader, China[18XD1403700] ; National Scientific and Technological Major Special Project of China[2018ZX09201008-002] ; Scientific Research Project of Shanghai University of Traditional Chinese Medicine, China[18TS087] ; Scientific Research Project of Shanghai University of Traditional Chinese Medicine, China[2018029] ; Shanghai Pujiang Program, China[2019PJD004]
WOS关键词	POGOSTEMONIS HERBA ; INDOMETHACIN ; MECHANISM ; INJURY ; MUCOSA
WOS研究方向	Immunology ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000548143500055
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/292095]
专题	中国科学院上海药物研究所
通讯作者	Wang, Zhi-cheng; Zhang, Tong; Wang, Bing
作者单位	1.Shanghai Univ Tradit Chinese Med, Expt Ctr Teaching & Learning, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Sch Pharm, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Foreign Languages Teaching Ctr, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, State Key Lab Drug Res, 501 Hai Ke Rd, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut Res, 501 Hai Ke Rd, Shanghai 201203, Peoples R China 6.Fudan Univ, Huashan Hosp, Dept Lab Med, 12 Wu Lu Mu Qi Middle Rd, Shanghai 200040, Peoples R China 7.Shanghai Univ Tradit Chinese Med, Shuguang Hosp, 528 Zhang Heng Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Xie, Lu,Guo, Yi-lin,Chen, Yu-rou,et al. A potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol-induced rat model[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2020,85:9.
APA	Xie, Lu.,Guo, Yi-lin.,Chen, Yu-rou.,Zhang, Li-ying.,Wang, Zhi-cheng.,...&Wang, Bing.(2020).A potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol-induced rat model.INTERNATIONAL IMMUNOPHARMACOLOGY,85,9.
MLA	Xie, Lu,et al."A potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol-induced rat model".INTERNATIONAL IMMUNOPHARMACOLOGY 85(2020):9.