Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes

doi:10.1007/s00216-020-02922-z

	Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes
	Li, Hao-Jv 1,2; Wei, Wen-Long 2; Li, Zhen-Wei 1,2; Yao, Chang-Liang 2; Wang, Meng-Yuan 1,2; Zhang, Jian-Qing 2; Li, Jia-Yuan 2; Guo, De-An 1,2
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2020-09-05
页码	7
关键词	Uncaria rhynchophylla Indole alkaloids Mass defect filter Time-staggered targeted ion lists Metabolite identification Liver microsomes
ISSN号	1618-2642
DOI	10.1007/s00216-020-02922-z
通讯作者	Guo, De-An(daguo@simm.ac.cn)
英文摘要	Metabolites have a close relationship with the efficacy and safety of herbal medicines. However, ubiquitous matrix interferences, complex co-elution, and minor or trace amounts in plasma restrict the comprehensive identification of metabolites. In this study, an efficient strategy comprising a mass defect filter and time-staggered targeted ion lists was established to characterize the metabolites of alkaloids ofUncaria rhynchophylla(UR) for the systematic comparison of metabolic differences in rat, mouse, dog, pig, monkey and human liver microsomes. The mass defect filter model effectively decreased interfering ions by 63-68%, and time-staggered precursor ion lists significantly increased the number of triggered MS/MS fragmentation by 65-120% in liver microsomes of six species. Ultimately, a total of 165 metabolites in the liver microsomes of six species were tentatively characterized, and the main metabolic pathways were demethylation, isomerization, hydrolysis, oxygenation and dehydrogenation. The results showed that the mouse liver microsomes exhibited metabolic behavior most similar to human metabolism of UR alkaloids. We hope that these results provide basic data for further investigation of UR metabolism in different species, and that the strategy can provide a reference for metabolite characterization of herbal medicines in complex biological matrix.
资助项目	National Key R&D Program of China[2019YFC1711000] ; National Natural Science Foundation of China[81530095] ; National Natural Science Foundation of China[81673591] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020348] ; National Standardization of Traditional Chinese Medicine Project[ZYBZH K LN 01] ; Science and Technology Commission Foundation of Shanghai[15DZ0502800] ; Guangdong Provincial Key R&D Plan in key priority fields[2020B1111110007]
WOS关键词	INTEGRATED STRATEGY ; LC-MS/MS ; ALKALOIDS ; ISORHYNCHOPHYLLINE ; PHARMACOKINETICS ; CLASSIFICATION ; IDENTIFICATION ; PHYTOCHEMISTRY ; ISOCORYNOXEINE ; PHARMACOLOGY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000566049300001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291586]
专题	中国科学院上海药物研究所
通讯作者	Guo, De-An
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Li, Hao-Jv,Wei, Wen-Long,Li, Zhen-Wei,et al. Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2020:7.
APA	Li, Hao-Jv.,Wei, Wen-Long.,Li, Zhen-Wei.,Yao, Chang-Liang.,Wang, Meng-Yuan.,...&Guo, De-An.(2020).Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,7.
MLA	Li, Hao-Jv,et al."Systematic comparison of metabolic differences of Uncaria rhynchophylla in rat, mouse, dog, pig, monkey and human liver microsomes".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020):7.