A novel pectin from Polygala tenuifolia blocks A beta(42) aggregation and production by enhancing insulin-degradation enzyme and neprilysin

doi:10.1016/j.ijbiomac.2020.05.212

	A novel pectin from Polygala tenuifolia blocks A beta(42) aggregation and production by enhancing insulin-degradation enzyme and neprilysin
	Zeng, Hui 1,2; Li, Piaopiao 2,3; Zhou, Lishuang 1,2; Ding, Kan 1,2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2020-10-15
卷号	161 页码:35-43
关键词	Polygala tenuifolia Pectin Polysaccharide A beta(42) Alzheimer's disease
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2020.05.212
通讯作者	Ding, Kan(dingkan@simm.ac.cn)
英文摘要	More and more evidences show that pectin polysaccharide may have impact on A beta(42). one important molecule implicated in Alzhemer's disease pathology. We speculate special structural motif of pectin might have better bioactivity on A beta(42). To address this hypothesis, we reported structure and impact of a novel pectin RP02-1 with the molecular weight of 116.0 kDa from roots of Polygala tenuifolia on A beta(42) aggregation and production and the underlying mechanism. Its structure is characterized as a backbone of alternate 1, 2, 4-linked alpha-Rhap and 1, 4-linked alpha-GalpA, with branches of terminal (T) -, 1, 3-,1, 4-, 1, 6- and 1, 3, 6-linked beta-Galp, T-, 1, 5- and 1, 3, 5-linked alpha-Araf substituted at C-4 of 1, 2,4-linked alpha-Rhap. Bioactivity study shows that this pectin may significantly block the aggregation of A beta(42). We further show that RP02-1 suppresses A beta(42) production with no apparent cytotoxicity in both CHO/APPBACE1 and HEK293-APPsw cells. Mechanism study demonstrates that RP02-1 may enhance the expression of insulin-degradation enzyme (IDE) and neprilysin (NEP), which are the main enzymes involved in Afi degradation. These results suggest that RP02-1 may be a candidate leading compound for anti-Alzheimer's disease new drug development by attenuating AN, production and inhibiting A beta(42) aggregation. (C) 2020 Elsevier B.V. All rights reserved.
资助项目	New Drug Creation and Manufacturing Program[2019ZX09735001] ; Ministry of Science and Technology ; People Republic of China ; National Natural Science Foundation of China[31670814] ; National Natural Science Foundation of China[31870801]
WOS关键词	ALZHEIMERS-DISEASE ; STRUCTURAL ELUCIDATION ; LONICERA-JAPONICA ; DEGRADING ENZYME ; TRANSGENIC MICE ; FLOWERS ; ARABINOGALACTAN ; PATHWAYS ; PROTEIN ; FRUITS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000571203600004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291221]
专题	中国科学院上海药物研究所
通讯作者	Ding, Kan
作者单位	1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 3.Nanchang Univ, Sch Basic Med, Nanchang 330006, Jiangxi, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Hui,Li, Piaopiao,Zhou, Lishuang,et al. A novel pectin from Polygala tenuifolia blocks A beta(42) aggregation and production by enhancing insulin-degradation enzyme and neprilysin[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,161:35-43.
APA	Zeng, Hui,Li, Piaopiao,Zhou, Lishuang,&Ding, Kan.(2020).A novel pectin from Polygala tenuifolia blocks A beta(42) aggregation and production by enhancing insulin-degradation enzyme and neprilysin.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,161,35-43.
MLA	Zeng, Hui,et al."A novel pectin from Polygala tenuifolia blocks A beta(42) aggregation and production by enhancing insulin-degradation enzyme and neprilysin".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 161(2020):35-43.