Osteogenic and antibacterial properties of vancomycin-laden mesoporous bioglass/PLGA composite scaffolds for bone regeneration in infected bone defects

doi:10.1080/21691401.2017.1396997

	Osteogenic and antibacterial properties of vancomycin-laden mesoporous bioglass/PLGA composite scaffolds for bone regeneration in infected bone defects
	Cheng, Tao 1; Qu, Haiyun 2; Zhang, Guoyou 3; Zhang, Xianlong 1
刊名	ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
	2018
卷号	46 期号:8 页码:1935-1947
关键词	Mesoporous bioactive glass infection antimicrobial osteogenesis osteomyelitis
ISSN号	2169-1401
DOI	10.1080/21691401.2017.1396997
通讯作者	Cheng, Tao(dr_tao.cheng@hotmail.com)
英文摘要	Chronic osteomyelitis and infected bone defects are substantial challenges faced by orthopaedic surgeons. In this study, vancomycin was loaded into mesoporous bioactive glass (MBG) to form a local antibiotic delivery system and then a bone tissue-engineering scaffold combining MBG and poly-(L-lactic-co-glycolic acid) (PLGA) was prepared by freeze-drying fabrication. In vitro degradation and water contact angle analysis suggested that the MBG-incorporated PLGA scaffold exhibited controlled degradability, stabilizing the pH of the surrounding environment and improved the hydrophilicity. Moreover, the presence of MBG provides a well-interconnected pore structure, to which human bone marrow-derived mesenchymal stem cells can attach, spread and proliferate, promoting upregulation of the expression of osteogenic markers. Thus, MBG/PLGA scaffolds exhibit better cytocompatibility and osteoblastic difierentiation properties compared with pure PLGA scaffolds. Vancomycin-loaded scaffolds have been found to yield sustained release that lasts for more than eight weeks in vitro. We tested the antibacterial performance of vancomycin-loaded scaffolds against Staphylococcus aureus, the most common bacteria isolated from infected bone. In vitro experiments demonstrated that loading vancomycin onto the scaffold promoted antibacterial activity and inhibited biofilm formation without deleterious effect on cytocompatibility. In conclusion, the novel inorganic-organic composites are considered potential materials for the treatment of infected bone defects.
资助项目	National Natural Science Foundation of China[81301571] ; Shanghai Municipal Health and Family Planning Commission for Distinguished Young Doctors[20141032]
WOS关键词	BIOACTIVE GLASS SCAFFOLDS ; IN-VITRO ; DELIVERY ; MICROSPHERES ; BIOMATERIALS
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering ; Materials Science
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000452031000039
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/290901]
专题	中国科学院上海药物研究所
通讯作者	Cheng, Tao
作者单位	1.Shanghai Jiao Tong Univ, Dept Orthopaed Surg, Affiliated Peoples Hosp 6, 600 Yishan Rd, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, Anal & Testing Ctr Inorgan Mat, Shanghai, Peoples R China 3.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Tao,Qu, Haiyun,Zhang, Guoyou,et al. Osteogenic and antibacterial properties of vancomycin-laden mesoporous bioglass/PLGA composite scaffolds for bone regeneration in infected bone defects[J]. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,2018,46(8):1935-1947.
APA	Cheng, Tao,Qu, Haiyun,Zhang, Guoyou,&Zhang, Xianlong.(2018).Osteogenic and antibacterial properties of vancomycin-laden mesoporous bioglass/PLGA composite scaffolds for bone regeneration in infected bone defects.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,46(8),1935-1947.
MLA	Cheng, Tao,et al."Osteogenic and antibacterial properties of vancomycin-laden mesoporous bioglass/PLGA composite scaffolds for bone regeneration in infected bone defects".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 46.8(2018):1935-1947.