Triple-Helix Conformation of a Polysaccharide Determined with Light Scattering, AFM, and Molecular Dynamics Simulation | |
Meng, Yan4; Shi, Xiaodan3; Cai, Liqin4; Zhang, Shihai2; Ding, Kan2; Nie, Shaoping3; Luo, Chuanfu1; Xu, Xiaojuan4; Zhang, Lina4 | |
刊名 | MACROMOLECULES |
2018-12-25 | |
卷号 | 51期号:24页码:10150-10159 |
ISSN号 | 0024-9297 |
DOI | 10.1021/acs.macromol.8b02017 |
通讯作者 | Luo, Chuanfu(luocf@ciac.ac.cn) ; Xu, Xiaojuan(xuxj@whu.edu.cn) ; Zhang, Lina(zhangln@whu.edu.cn) |
英文摘要 | The chain conformation of a beta-glucan extracted from black fungus (BFP) was studied by static/dynamic light scattering, viscometry, atomic force microscopy (AFM), and molecular dynamics (MD) simulation. The Mark-Houwink equation and the relationship between M-w and R-g of BFP in water at 25 degrees C were determined to be [eta] = 1.78 X 10(-7)M(w)(1.6) and R-g = 5 x 10(-4)M(w)(0.9), and the molar mass per unit contour length (M-L) and the persistence length (q) were 2724 +/- 276 nm(-1) and 230 +/- 30 nm, respectively, indicating triple-helix conformation. Moreover, the stiff-chain lengths of the BFP fractions were visualized with AFM images, and their M-L values were estimated to give a mean of 2212 nm(-1), consistent with the above. Importantly, MD simulation confirmed that the triple helix was the most stable conformation of BFP. We identified, for the first time, the triple-helix chain conformation of BFP and also offered an alternative method for the characterization of the rigid macromolecules. |
资助项目 | Major Program of National Natural Science Foundation of China[21334005] ; Major International (Regional) Joint Research Project of National Natural Science Foundation of China[21620102004] ; National Natural Science Foundation of China[21574102] ; National Natural Science Foundation of China[21875167] ; National Key Research and Development Plan of China[2016YFD0400202] |
WOS关键词 | SCHIZOPHYLLUM-COMMUNE POLYSACCHARIDE ; DOUBLE-STRANDED HELIX ; CHAIN CONFORMATION ; HYDRODYNAMIC PROPERTIES ; INTRINSIC-VISCOSITY ; DILUTE-SOLUTION ; BETA-GLUCAN ; XANTHAN ; DEGRADATION ; LENTINAN |
WOS研究方向 | Polymer Science |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000454567400012 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/290857] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Luo, Chuanfu; Xu, Xiaojuan; Zhang, Lina |
作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Materia Med, Shanghai, Peoples R China 3.Nanchang Univ, Coll Food Sci, Nanchang, Jiangxi, Peoples R China 4.Wuhan Univ, Coll Chem & Mol Sci, Wuhan, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Meng, Yan,Shi, Xiaodan,Cai, Liqin,et al. Triple-Helix Conformation of a Polysaccharide Determined with Light Scattering, AFM, and Molecular Dynamics Simulation[J]. MACROMOLECULES,2018,51(24):10150-10159. |
APA | Meng, Yan.,Shi, Xiaodan.,Cai, Liqin.,Zhang, Shihai.,Ding, Kan.,...&Zhang, Lina.(2018).Triple-Helix Conformation of a Polysaccharide Determined with Light Scattering, AFM, and Molecular Dynamics Simulation.MACROMOLECULES,51(24),10150-10159. |
MLA | Meng, Yan,et al."Triple-Helix Conformation of a Polysaccharide Determined with Light Scattering, AFM, and Molecular Dynamics Simulation".MACROMOLECULES 51.24(2018):10150-10159. |
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