Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT

doi:10.1038/s41467-019-09649-1

	Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT
	Liu, Yuwei 5,6; Xue, Mengzhu 5; Du, Shaowei 5,7; Feng, Wanwan 8; Zhang, Ke 8; Zhang, Liwen 6,9,10; Liu, Haiyue 5,6; Jia, Guoyi 5,7; Wu, Lingshuang 1; Hu, Xin 2,11
刊名	NATURE COMMUNICATIONS
	2019-04-09
卷号	10 页码:12
ISSN号	2041-1723
DOI	10.1038/s41467-019-09649-1
通讯作者	Hu, Xin(xinhu@fudan.edu.cn) ; Chen, Luonan(lnchen@sibs.ac.cn) ; Wang, Peng(wangpeng@picb.ac.cn)
英文摘要	The competitive endogenous RNA (ceRNA) hypothesis suggests an intrinsic mechanism to regulate biological processes. However, whether the dynamic changes of ceRNAs can modulate miRNA activities remains controversial. Here, we examine the dynamics of ceRNAs during TGF-beta-induced epithelial-to-mesenchymal transition (EMT). We observe that TGFBI, a transcript highly induced during EMT in A549 cells, acts as the ceRNA for miR-21 to modulate EMT. We further identify FN1 as the ceRNA for miR-200c in the canonical SNAIL-ZEB-miR200 circuit in MCF10A cells. Experimental assays and computational simulations demonstrate that the dynamically induced ceRNAs are directly coupled with the canonical double negative feedback loops and are critical to the induction of EMT. These results help to establish the relevance of ceRNA in cancer EMT and suggest that ceRNA is an intrinsic component of the EMT regulatory circuit and may represent a potential target to disrupt EMT during tumorigenesis.
资助项目	National Key R&D Program of China[2017YFA0505500] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB13040700] ; National Natural Science Foundation of China[31271413] ; National Natural Science Foundation of China[31671380] ; National Natural Science Foundation of China[31771476]
WOS关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; TGF-BETA ; CERNA HYPOTHESIS ; MIR-200 FAMILY ; UP-REGULATION ; MICRORNA ; CANCER ; PROMOTES ; TRANSCRIPTION ; EXPRESSION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000463872400013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/290068]
专题	中国科学院上海药物研究所
通讯作者	Hu, Xin; Chen, Luonan; Wang, Peng
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Fudan Univ, Precis Canc Med Ctr, Shanghai Canc Ctr, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China 4.Res Ctr Brain Sci & Brain Inspired Intelligence, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Syst Biol, Shanghai 200031, Peoples R China 6.Univ Chinese Acad Sci, Shanghai 200031, Peoples R China 7.Shanghai Univ, Sch Life Sci, Shanghai 200031, Peoples R China 8.Chinese Acad Sci, Biomed Big Data Ctr, CAS Key Lab Computat Biol,Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol,Shanghai Inst, Shanghai 200031, Peoples R China 9.Chinese Acad Sci, Key Lab Syst Biol, Ctr Excellence Mol Cell Sci, Inst Biochem & Cell Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 10.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuwei,Xue, Mengzhu,Du, Shaowei,et al. Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT[J]. NATURE COMMUNICATIONS,2019,10:12.
APA	Liu, Yuwei.,Xue, Mengzhu.,Du, Shaowei.,Feng, Wanwan.,Zhang, Ke.,...&Wang, Peng.(2019).Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT.NATURE COMMUNICATIONS,10,12.
MLA	Liu, Yuwei,et al."Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT".NATURE COMMUNICATIONS 10(2019):12.