G-quadruplex and duplex DNA binding studies of novel Ruthenium(II) complexes containing ascididemin ligands

doi:10.1016/j.jinorgbio.2019.03.021

	G-quadruplex and duplex DNA binding studies of novel Ruthenium(II) complexes containing ascididemin ligands
	Wumaier, Maierhaba 1; Shi, Jing-Jing 2; Yao, Tian-Ming 1; Hu, Xiao-Chun 1; Gao, Ru-Ru 1; Shi, Shuo 1
刊名	JOURNAL OF INORGANIC BIOCHEMISTRY
	2019-07-01
卷号	196 页码:10
关键词	Ruthenium complexes G-quadruplex Duplex DNA Ascididemin
ISSN号	0162-0134
DOI	10.1016/j.jinorgbio.2019.03.021
通讯作者	Shi, Jing-Jing(shijingjing@simm.ac.cn) ; Yao, Tian-Ming(tmyao@tongji.edu.cn) ; Shi, Shuo(shishuo@tongji.edu.cn)
英文摘要	In this paper, three new Ruthenium(II) polypyridyl complexes containing ascididemin (ASC) as main ligand have been synthesized and characterized. Their interactions with different G-quadruplex (Htelo, c-myc and c-kit) (Htelo: human telomeric DNA, c-myc: cellular-myelocytomatosis viral oncogene, c-kit: oncogene c-kit promoter sequences) and duplex (ds26) DNA sequences were comparatively studied with the free ligand ASC by a series of spectroscopic techniques including UV-vis (ultraviolet-visible) spectroscopy, FID (fluorescent intercalator displacement) assay, and FRET (fluorescence resonance energy transfer) melting assay. Molecular docking studies were also performed to support the binding mode of the compounds with G-quadruplex DNA. Results indicated that [Ru(bpy)(2)ASC]center dot(PF6)(2) (1), [Ru(phen)(2)ASC]center dot(PF6)(2) (2), [Ru(tatp)(2)ASC]center dot(PF6)(2) (3) (bpy 2,2'-bipyridine, = phen = 1,10-phenanthroline, tatp = 1,4,8,9-tetra-aza-triphenylene) and ASC can effectively bind G-quadruplex and duplex DNA and stabilization ability lies in the order 3 > 2 > 1 > ASC. Complex 3 was determined to be the most promising candidate for further in vitro studies and potential anticancer drug.
资助项目	National Natural Science Foundation of China[21671150] ; National Natural Science Foundation of China[21877084] ; National Natural Science Foundation of China[81871730] ; National Natural Science Foundation of China[21472139] ; Science and Technology Commission of Shanghai Municipality[14DZ2261100] ; Fundamental Research Funds for the Central Universities
WOS关键词	MOLECULAR LIGHT SWITCH ; POLYPYRIDYL COMPLEXES ; METAL-COMPLEXES ; BIOLOGICAL EVALUATION ; TELOMERASE ACTIVITY ; SQUARE-PLANAR ; IN-VITRO ; PHASE-I ; STABILIZATION ; INHIBITION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000469888100003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/289653]
专题	中国科学院上海药物研究所
通讯作者	Shi, Jing-Jing; Yao, Tian-Ming; Shi, Shuo
作者单位	1.Tongji Univ, Sch Chem Sci & Engn, Shanghai Key Lab Chem Assessment & Sustainabil, Shanghai 200092, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, VARI SIMM Ctr,Ctr Struct & Funct Drug Targets, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wumaier, Maierhaba,Shi, Jing-Jing,Yao, Tian-Ming,et al. G-quadruplex and duplex DNA binding studies of novel Ruthenium(II) complexes containing ascididemin ligands[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2019,196:10.
APA	Wumaier, Maierhaba,Shi, Jing-Jing,Yao, Tian-Ming,Hu, Xiao-Chun,Gao, Ru-Ru,&Shi, Shuo.(2019).G-quadruplex and duplex DNA binding studies of novel Ruthenium(II) complexes containing ascididemin ligands.JOURNAL OF INORGANIC BIOCHEMISTRY,196,10.
MLA	Wumaier, Maierhaba,et al."G-quadruplex and duplex DNA binding studies of novel Ruthenium(II) complexes containing ascididemin ligands".JOURNAL OF INORGANIC BIOCHEMISTRY 196(2019):10.