Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins

doi:10.1002/chem.201903190

	Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins
	Xue, Dongxiang 1,2,3,4; Xu, Tiandan 1,3,4,5; Wang, Huixia 1,3,4,5; Wu, Meng 1,3,4,5; Yuan, Ya 1; Wang, Wei 6; Tan, Qiwen 1; Zhao, Fei 1; Zhou, Fang 1,2,3,4; Hu, Tao 1,3,4,5
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2019-08-13
页码	7
关键词	detergents disulfides membrane proteins micelle size NMR spectroscopy
ISSN号	0947-6539
DOI	10.1002/chem.201903190
通讯作者	Wuthrich, Kurt(wuthrich@scripps.edu) ; Tao, Houchao(taohch@shanghaitech.edu.cn)
英文摘要	Disulfide-containing detergents (DCDs) are introduced, which contain a disulfide bond in the hydrophobic tail. DCDs form smaller micelles than corresponding detergents with linear hydrocarbon chains, while providing good solubilization and reconstitution of membrane proteins. The use of this new class of detergents in structural biology is illustrated with solution NMR spectra of the human G protein-coupled receptor A(2A)AR, which is an alpha-helical protein, and the beta-barrel protein OmpX from E. coli.
资助项目	National Natural Science Foundation of China[21672147] ; National Natural Science Foundation of China[31670733] ; Shanghai Municipal Government, ShanghaiTech University
WOS关键词	CRYSTAL-STRUCTURE ; RECEPTOR ; NMR ; AMPHIPHILES ; STABILITY ; MICELLES ; COMPLEX ; ACIDS ; BILE
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000481113100001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/288921]
专题	中国科学院上海药物研究所
通讯作者	Wuthrich, Kurt; Tao, Houchao
作者单位	1.ShanghaiTech Univ, iHuman Inst, Ren Bldg,393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 6.Wuhan Univ, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Xue, Dongxiang,Xu, Tiandan,Wang, Huixia,et al. Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins[J]. CHEMISTRY-A EUROPEAN JOURNAL,2019:7.
APA	Xue, Dongxiang.,Xu, Tiandan.,Wang, Huixia.,Wu, Meng.,Yuan, Ya.,...&Tao, Houchao.(2019).Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins.CHEMISTRY-A EUROPEAN JOURNAL,7.
MLA	Xue, Dongxiang,et al."Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins".CHEMISTRY-A EUROPEAN JOURNAL (2019):7.