Profiling and identification of aqueous extract of Cordyceps sinensis by ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry

	Profiling and identification of aqueous extract of Cordyceps sinensis by ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry
	Yao Chang-Liang 2,3; Qian Zheng-Ming 1; Tian Wen-Shuai 2; Xu Xiao-Qian 2; Yan Yu 2; Shen Yao 2; Lu Song-Mao 2; Li Wen-Jia 1; Guo De-An 2,3
刊名	CHINESE JOURNAL OF NATURAL MEDICINES
	2019-08-01
卷号	17 期号:8 页码:631-640
关键词	Aqueous extract Cordyceps sinensis Dipeptides Nucleosides
ISSN号	2095-6975
通讯作者	Li Wen-Jia(liwenjiapa-per@163.com) ; Guo De-An(daguo@simm.ac.cn)
英文摘要	Characterization of aqueous extract in traditional Chinese medicine (TCM) is challenging due to the poor retention of the analytes on conventional C-18 columns. This study presents a systematic characterization method based on a rapid chromatographic separation (8 min) on a polar-modified C-18 (Waters Cortecs T3) column of aqueous extract of Cordyceps sinensis. UHPLC-HRMS method was used to profile components in both untargeted and targeted manners by full MS/PIL/dd-MS2 acquisition approach. The components were identified or tentatively identified by reference standards comparison, fragmentation rules elucidation and available databases search. A total of 91 components, including 10 nucleobases, 20 nucleosides, 39 dipeptides, 18 amino acids and derivatives and 4 other components, were characterized from the aqueous extract of C. sinensis. And this was the first time to systematically report the presence of nucleosides and dipeptides in C. sinensis, especially for modified nucleosides. The chemical basis inquiry of this work would be beneficial to mechanism exploration and quality control of C. sinensis and related products. Meanwhile, this work also provided an effective solution for characterization of aqueous extract in TCM.
资助项目	National Key R&D Program of China[2018YFC1707900] ; National Key R&D Program of China[2018YFC1707300]
WOS关键词	ACIDS ; NUCLEOBASES ; NUCLEOSIDES ; COMPONENTS ; TOOL
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
语种	英语
出版者	CHINESE JOURNAL NATURAL MEDICINES
WOS记录号	WOS:000483457100009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/288789]
专题	中国科学院上海药物研究所
通讯作者	Li Wen-Jia; Guo De-An
作者单位	1.Sunshine Lake Pharma Co Ltd, Key Lab State Adm Tradit Chinese Med, Dongguan 523850, Guangdong, Peoples R China 2.GenChim Testing Shanghai Co Ltd, R&D Dept, Shanghai 200131, Peoples R China 3.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yao Chang-Liang,Qian Zheng-Ming,Tian Wen-Shuai,et al. Profiling and identification of aqueous extract of Cordyceps sinensis by ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2019,17(8):631-640.
APA	Yao Chang-Liang.,Qian Zheng-Ming.,Tian Wen-Shuai.,Xu Xiao-Qian.,Yan Yu.,...&Guo De-An.(2019).Profiling and identification of aqueous extract of Cordyceps sinensis by ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry.CHINESE JOURNAL OF NATURAL MEDICINES,17(8),631-640.
MLA	Yao Chang-Liang,et al."Profiling and identification of aqueous extract of Cordyceps sinensis by ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry".CHINESE JOURNAL OF NATURAL MEDICINES 17.8(2019):631-640.