Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP

doi:10.15252/embj.2018100948

	Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP
	Xu, Zhaowei 1; Zhang, Hainan 1; Zhang, Xingrun 2,3; Jiang, Hewei 1; Liu, Chengxi 1; Wu, Fanlin 1; Qian, Lili 4,5; Hao, Bingbing 4,5; Czajkowsky, Daniel M.6; Guo, Shujuan 1
刊名	EMBO JOURNAL
	2019-08-16
页码	18
关键词	c-di-GMP CobB diguanylate cyclase negative feedback loop protein acetylation
ISSN号	0261-4189
DOI	10.15252/embj.2018100948
通讯作者	Tao, Sheng-ce(taosc@sjtu.edu.cn)
英文摘要	As a ubiquitous bacterial secondary messenger, c-di-GMP plays key regulatory roles in processes such as bacterial motility and transcription regulation. CobB is the Sir2 family protein deacetylase that controls energy metabolism, chemotaxis, and DNA supercoiling in many bacteria. Using an Escherichia coli proteome microarray, we found that c-di-GMP strongly binds to CobB. Further, protein deacetylation assays showed that c-di-GMP inhibits the activity of CobB and thereby modulates the biogenesis of acetyl-CoA. Interestingly, we also found that one of the key enzymes directly involved in c-di-GMP production, DgcZ, is a substrate of CobB. Deacetylation of DgcZ by CobB enhances its activity and thus the production of c-di-GMP. Our work establishes a novel negative feedback loop linking c-di-GMP biogenesis and CobB-mediated protein deacetylation.
资助项目	National Key Research and Development Program of China[2016YFA0500600] ; National Natural Science Foundation of China[31670831] ; National Natural Science Foundation of China[31370813] ; National Natural Science Foundation of China[31370750] ; National Natural Science Foundation of China[31670722] ; National Natural Science Foundation of China[31670066] ; National Basic Research Program of China (973 Program)[2014CBA02004] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program[2018ZX09711002-004]
WOS关键词	ESCHERICHIA-COLI ; SIRTUIN DEACETYLASE ; ACETYLATION ; MECHANISM ; PHOSPHODIESTERASE ; QUANTIFICATION ; IDENTIFICATION ; METABOLISM ; REVEALS ; DOMAIN
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000481589400001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/288530]
专题	中国科学院上海药物研究所
通讯作者	Tao, Sheng-ce
作者单位	1.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai, Peoples R China 2.Tsinghua Univ, Ctr Struct Biol, Sch Life Sci, MOE Key Lab Prot Sci, Beijing, Peoples R China 3.Tsinghua Univ, Sch Med, Dept Basic Med Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Chem Prote Ctr, Shanghai, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai, Peoples R China 7.Shanghai Normal Univ, Coll Life Sci, Shanghai, Peoples R China 8.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Key Lab Noncoding RNA, Beijing, Peoples R China 9.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing, Peoples R China 10.Foshan Univ, Sch Stomatol & Med, Foshan, Peoples R China
推荐引用方式 GB/T 7714	Xu, Zhaowei,Zhang, Hainan,Zhang, Xingrun,et al. Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP[J]. EMBO JOURNAL,2019:18.
APA	Xu, Zhaowei.,Zhang, Hainan.,Zhang, Xingrun.,Jiang, Hewei.,Liu, Chengxi.,...&Tao, Sheng-ce.(2019).Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP.EMBO JOURNAL,18.
MLA	Xu, Zhaowei,et al."Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP".EMBO JOURNAL (2019):18.