trimerprocyanidinoligomerscontributetotheprotectiveeffectsofcinnamonextractsonpancreaticcellsinvitro

doi:10.1038/aps.2016.29

	trimerprocyanidinoligomerscontributetotheprotectiveeffectsofcinnamonextractsonpancreaticcellsinvitro
	Sun Peng 1; Wang Ting 1; Chen Lu 2; Yu Bangwei 1; Jia Qi 2; Chen Kaixian 1; Fan Huimin 3; Li Yiming 2; Wang Heyao 1
刊名	actapharmacologicasinica
	2016
卷号	37 期号:8 页码:1083
关键词	cinnamon procyanidin oligomers cinnamtannin B1 procyanidin C1 cinnamtannin D1 diabetes pancreatic β-cells cultured murine islets lipotoxicity ROS insulin secretion
ISSN号	1671-4083
DOI	10.1038/aps.2016.29
英文摘要	Aim: Cinnamon extracts rich in procyanidin oligomers have shown to improve pancreatic β-cell function in diabetic db/db mice. The aim of this study was to identify the active compounds in extracts from two species of cinnamon responsible for the pancreatic β-cell protection in vitro. Methods: Cinnamon extracts were prepared from Cinnamomum tamala(CT-E) and Cinnamomum cassia(CC-E). Six compounds procyanidin B2(cpd1),(–)-epicatechin(cpd2), cinnamtannin B1(cpd3), procyanidin C1(cpd4), parameritannin A1(cpd5) and cinnamtannin D1(cpd6) were isolated from the extracts. INS-1 pancreatic β-cells were exposed to palmitic acid(PA) or H_2O_2 to induce lipotoxicity and oxidative stress. Cell viability and apoptosis as well as ROS levels were assessed. Glucose-stimulated insulin secretion was examined in PA-treated β-cells and murine islets. Results: CT-E, CC-E as well as the compounds, except cpd5, did not cause cytotoxicity in the β-cells up to the maximum dosage using in this experiment. CT-E and CC-E(12.5–50 μg/mL) dose-dependently increased cell viability in both PA- and H_2O_2-treated β-cells, and decreased ROS accumulation in H_2O_2-treated β-cells. CT-E caused more prominent β-cell protection than CC-E. Furthermore, CT-E(25 and 50 μg/mL) dose-dependently increased glucose-stimulated insulin secretion in PA-treated β-cells and murine islets, but CC-E had little effect. Among the 6 compounds, trimer procyanidins cpd3, cpd4 and cpd6(12.5–50 μmol/L) dose-dependently increased the cell viability and decreased ROS accumulation in H_2O_2-treated β-cells. The trimer procyanidins also increased glucose-stimulated insulin secretion in PA-treated β-cells. Conclusion: Trimer procyanidins in the cinnamon extracts contribute to the pancreatic β-cell protection, thus to the anti-diabetic activity.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/285321]
专题	中国科学院上海药物研究所
作者单位	1.中国科学院上海药物研究所 2.上海中医药大学 3.同济大学
推荐引用方式 GB/T 7714	Sun Peng,Wang Ting,Chen Lu,et al. trimerprocyanidinoligomerscontributetotheprotectiveeffectsofcinnamonextractsonpancreaticcellsinvitro[J]. actapharmacologicasinica,2016,37(8):1083.
APA	Sun Peng.,Wang Ting.,Chen Lu.,Yu Bangwei.,Jia Qi.,...&Wang Heyao.(2016).trimerprocyanidinoligomerscontributetotheprotectiveeffectsofcinnamonextractsonpancreaticcellsinvitro.actapharmacologicasinica,37(8),1083.
MLA	Sun Peng,et al."trimerprocyanidinoligomerscontributetotheprotectiveeffectsofcinnamonextractsonpancreaticcellsinvitro".actapharmacologicasinica 37.8(2016):1083.