Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity

doi:10.1021/acs.analchem.9b03600

	Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity
	Ding, Chunyong 2; Wang, Fengyang 1; Dang, Yijing 1; Xu, Zhiai 1; Li, Lingling 1; Lai, Yi 1; Yu, Haijun 3; Luo, Yi 4; Huang, Ruimin 3; Zhang, Ao 2
刊名	ANALYTICAL CHEMISTRY
	2019-12-17
卷号	91 期号:24 页码:15577-15584
ISSN号	0003-2700
DOI	10.1021/acs.analchem.9b03600
通讯作者	Ding, Chunyong(chding@simm.ac.cn) ; Xu, Zhiai(zaxu@chem.ecnu.edu.cn) ; Zhang, Wen(wzhang@chem.ecnu.edu.cn)
英文摘要	The accurate detection of tumorous methylglyoxal (MGO) and its detoxifier glyoxalase 1 (GLO1) in living systems is critical for understanding their roles in tumor initiation and progression. To date, the in situ fluorescence detection of endogenous MGO and GLO1 in tumor has not been reported. Herein we developed a near-infrared (NIR) fluorescent probe MEBTD to specifically detect tumorous MGO. Compared with previously reported MGO fluorescent probes, MEBTD exhibits several distinct advantages, including NIR emission, high selectivity with an MGO detection limit of 18 nM, and a 131-fold off-on ratio. The probe could sense GLO1 activity and monitor the therapeutic effect of GLO1 inhibitors by imaging tumorous MGO in a both a real-time and in situ manner, demonstrating that the biological effect of GLO1 inhibitors is dependent on the GLO1 activity. Furthermore, MEBTD enables the visualization of tumorous MGO induced by GLO1 inhibitors in vivo. To the best of our knowledge, MEBTD is the first NIR fluorescent probe for specifically imaging tumorous MGO in living animals, indicating the promising potential for tumor diagnosis and therapeutic evaluation.
资助项目	National Natural Science Foundation of China[21675055] ; National Natural Science Foundation of China[81402790] ; National Natural Science Foundation of China[21775046] ; National Natural Science Foundation of China[21635003] ; Personalized Medicines Molecular Signature-based Drug Discovery and Development of the Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020320] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002] ; State Key Laboratory of Drug Research[SIMM1903KF-13] ; One Hundred Talent Program of the Chinese Academy of Sciences
WOS关键词	ALZHEIMERS-DISEASE ; I INHIBITION ; APOPTOSIS ; PLASMA ; 3-DEOXYGLUCOSONE ; MECHANISM ; GLYCATION ; OBESITY ; STRESS ; CELLS
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000503910600034
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/282295]
专题	中国科学院上海药物研究所
通讯作者	Ding, Chunyong; Xu, Zhiai; Zhang, Wen
作者单位	1.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Dalian Univ Technol, Sch Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
推荐引用方式 GB/T 7714	Ding, Chunyong,Wang, Fengyang,Dang, Yijing,et al. Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity[J]. ANALYTICAL CHEMISTRY,2019,91(24):15577-15584.
APA	Ding, Chunyong.,Wang, Fengyang.,Dang, Yijing.,Xu, Zhiai.,Li, Lingling.,...&Zhang, Wen.(2019).Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity.ANALYTICAL CHEMISTRY,91(24),15577-15584.
MLA	Ding, Chunyong,et al."Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity".ANALYTICAL CHEMISTRY 91.24(2019):15577-15584.