Design, Synthesis and Bioactivity of Core 1 O-glycan and its Derivative on Human Gut Microbiota

doi:10.2174/1570180816666181218143207

	Design, Synthesis and Bioactivity of Core 1 O-glycan and its Derivative on Human Gut Microbiota
	Qu, Huanhuan 2; Li, Baixue 3; Yang, Jingyi 1; Liang, Huaiwen 1; Li, Meixia 2; Ding, Kan 2
刊名	LETTERS IN DRUG DESIGN & DISCOVERY
	2019
卷号	16 期号:12 页码:1348-1353
关键词	Bioactivity core 1 derivative human gut microbiota O-glycan synthesis
ISSN号	1570-1808
DOI	10.2174/1570180816666181218143207
通讯作者	Qu, Huanhuan(quhuanhuan@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn)
英文摘要	Background: Disaccharide core 1 (Gal beta 1-3GalNAc) is a common O-glycan structure in nature. Biochemical studies have confirmed that the formation of the core 1 structure is an important initial step in O-glycan biosynthesis and it is of great importance for human body. Objective: Our study will provide meaningful and useful sights for O-glycan synthesis and their bioassay. And all the synthetic glycosides would be used as intermediate building blocks in the scheme developed for oligosaccharide construction. Methods: In this article, we firstly used chemical procedures to prepare core 1 and its derivative, and a novel disaccharide was efficiently synthesized. The structures of the synthesized compounds were elucidated and confirmed by H-1 NMR, C-13 NMR and MS. Then we employed three human gut symbionts belonging to Bacteroidetes, a predominantphyla in the distal gut, as models to study the bioactivity of core 1 and its derivative on human gut microbiota. Results: According to our results, both core 1 and derivative could support the growth of B. fragilis, especially the core 1 derivative, while failed to support the growth of B. thetaiotaomicron and B. ovatus. Conclusion: This suggested that the B. fragilis might have the specificity glycohydrolase to cut the glycosidic bond for acquiring monosaccharide.
资助项目	National Natural Science Foundation of China (NSFC)[81603530] ; National Natural Science Foundation of China (NSFC)[21402224] ; Shanghai Science and Technology Development Funds[14YF1407800]
WOS关键词	CHEMOENZYMATIC SYNTHESIS ; SUPPLEMENTATION ; DIGESTIBILITY ; PERFORMANCE ; ANTIGEN
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000496232800005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281855]
专题	中国科学院上海药物研究所
通讯作者	Qu, Huanhuan; Ding, Kan
作者单位	1.Shanghai Univ Tradit Chinese Med, Dept Pharm, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 3.Chengdu Univ Tradit Chinese Med, Dept Basic Mediclal Sci, Chengdu 610075, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Qu, Huanhuan,Li, Baixue,Yang, Jingyi,et al. Design, Synthesis and Bioactivity of Core 1 O-glycan and its Derivative on Human Gut Microbiota[J]. LETTERS IN DRUG DESIGN & DISCOVERY,2019,16(12):1348-1353.
APA	Qu, Huanhuan,Li, Baixue,Yang, Jingyi,Liang, Huaiwen,Li, Meixia,&Ding, Kan.(2019).Design, Synthesis and Bioactivity of Core 1 O-glycan and its Derivative on Human Gut Microbiota.LETTERS IN DRUG DESIGN & DISCOVERY,16(12),1348-1353.
MLA	Qu, Huanhuan,et al."Design, Synthesis and Bioactivity of Core 1 O-glycan and its Derivative on Human Gut Microbiota".LETTERS IN DRUG DESIGN & DISCOVERY 16.12(2019):1348-1353.