Global characterization of proteome and lysine methylome features in EZH2 wild-type and mutant lymphoma cell lines

doi:10.1016/j.jprot.2019.103614

	Global characterization of proteome and lysine methylome features in EZH2 wild-type and mutant lymphoma cell lines
	Hao, Bingbing 1,3; Sun, Mingwei 1,2; Zhang, Min 1; Zhao, Xiaoxiao 1; Zhao, Lei 1; Li, Bolin 1; Zhai, Linhui 1; Liu, Ping 1; Hu, Hao 1; Xu, Jun-Yu 1
刊名	JOURNAL OF PROTEOMICS
	2020-02-20
卷号	213 页码:10
关键词	Lysine methylome Quantitative proteomics EZH2 Diffuse large B cell lymphoma PTM crosstalk
ISSN号	1874-3919
DOI	10.1016/j.jprot.2019.103614
通讯作者	Xu, Jun-Yu(jyxu@simm.ac.cn) ; Tan, Minjia(mjtan@simm.ac.cn)
英文摘要	Lysine methylation is a widespread protein post-translational modification showing essentialities in versatile cellular process. EZH2, a methyltransferase specifically trimethylates the lysine 27 of histone H3 and its aberrance in several cancers promotes the development of its inhibitors against hematological tumors. In this study, we presented a deep exploration of lysine mono-, di- and trimethylomes in EZH2 wild-type and Y641 mutant lymphoma cell lines. Our results showed that several substrates were modified in different methylation levels. Moreover, these methylated lysine residues could also undergo other types of PTMs. Combined with the differences proved in protein expression, lysine acetylation, lysine ubiquitylation and protein N-termianl acetylation level, our study underlined the substrate specificity of lysine methylation and its crosstalk with other types of PTMs. Totally, our study raised new insights into the global cellular methylation features in hematological cell lines, which provided further inspects into the distribution and function of lysine methylation. Significance: Our study showed the global landscape of mono-, di- and trimethylomes in the EZH2-aberrant DLBCL cell lines, revealing the molecular characteristics of lysine methylation. Combined with the protein abundance and potential crosstalk among different types of PTMs, our study raised new insights into the global cellular methylation features in hematological tumors and provided further inspects into the distribution and function of lysine methylation.
资助项目	Natural Science Foundation of China[91753203] ; Natural Science Foundation of China[81872888] ; Natural Science Foundation of China[91853126] ; Natural Science Foundation of China[31670066] ; K. C. Wong Education Foundation ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12050406] ; National Science and Technology Major Project Key New Drug Creation and Manufacturing Program[2018ZX09711002-004] ; China Postdoctoral Science Foundation[2019T120368] ; Shanghai Sailing Program[19YF1457700] ; SA-SIBS Scholarship Program (2018)
WOS关键词	HISTONE METHYLTRANSFERASE EZH2 ; POSTTRANSLATIONAL MODIFICATIONS ; METHYLATION ; HYPERTRIMETHYLATION ; MUTATIONS ; STRATEGY ; TARGET ; CANCER ; ROLES ; H3
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000510957000012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281681]
专题	中国科学院上海药物研究所
通讯作者	Xu, Jun-Yu; Tan, Minjia
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Guangzhou Regenerat Med & Hlth Guangdong Lab, Guangzhou 510005, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hao, Bingbing,Sun, Mingwei,Zhang, Min,et al. Global characterization of proteome and lysine methylome features in EZH2 wild-type and mutant lymphoma cell lines[J]. JOURNAL OF PROTEOMICS,2020,213:10.
APA	Hao, Bingbing.,Sun, Mingwei.,Zhang, Min.,Zhao, Xiaoxiao.,Zhao, Lei.,...&Tan, Minjia.(2020).Global characterization of proteome and lysine methylome features in EZH2 wild-type and mutant lymphoma cell lines.JOURNAL OF PROTEOMICS,213,10.
MLA	Hao, Bingbing,et al."Global characterization of proteome and lysine methylome features in EZH2 wild-type and mutant lymphoma cell lines".JOURNAL OF PROTEOMICS 213(2020):10.