Molecular dynamics simulation revealed the intrinsic conformational change of cellular inhibitor of apoptosis protein-1

doi:10.1080/07391102.2019.1591303

	Molecular dynamics simulation revealed the intrinsic conformational change of cellular inhibitor of apoptosis protein-1
	Xu, Heng 1,2; Tang, Zhengkuan 3; Zuo, Yu 4; Xiong, Fengmin 4; Chen, Kaixian 1,2; Jiang, Hualiang 1,2; Luo, Cheng 1,2; Zhang, Hao 1,2
刊名	JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
	2020-03-03
卷号	38 期号:4 页码:975-984
关键词	cIAP1 dimerization conformational change MD simulation
ISSN号	0739-1102
DOI	10.1080/07391102.2019.1591303
通讯作者	Luo, Cheng(cluo@simm.ac.cn) ; Zhang, Hao(hao_cadd@simm.ac.cn)
英文摘要	Inhibitor of apoptosis proteins (IAPs) are important regulators of apoptosis, and protein targets for the development of anti-cancer drugs. Cellular inhibitor of apoptosis protein-1 (cIAP1) is an important member of IAPs. Peptides or small-molecular antagonists can induce the dimerization, auto-ubiquitination, and proteasomal degradation of the cellular inhibitor of apoptosis protein-1 (cIAP1). While in the absence of antagonists, several mutations of the cIAP1 protein also lead to its dimerization and auto-ubiquitination. Even though the crystal structure of cIAP1 protein has been determined, the intrinsic mechanism of its dimerization remains unexplored. Accumulating evidence indicated that intrinsic conformational change existed during the binding of antagonists with cIAP1 protein, or introduction of mutations. To reveal this intrinsic conformational change, molecular dynamics simulations at microsecond scale were applied for the wild-type and mutant-type cIAP1 proteins. Compared to the crystal structure, significant conformational change was observed during the simulations, which could explain the importance of previously identified key mutations. To validate these findings revealed by our simulations, a new mutation D303A was constructed and the following native polyacrylamide gel electrophoresis (native-PAGE) assay observed a proportion of spontaneous dimerization, in comparison with the wild-type control. Taken together, these computational and experimental results revealed the intrinsic conformational change of cIAP1, which could not only explain previously identified key mutations, but also be exploited for further design and development of anti-tumor compounds that target the cIAP1 protein. Communicated by Ramaswamy H. Sarma
WOS关键词	NF-KAPPA-B ; CANCER-CELLS ; ANTAGONISTS INDUCE ; FORCE-FIELD ; C-IAPS ; CIAP1 ; LIGASES
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000517395200002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281218]
专题	中国科学院上海药物研究所
通讯作者	Luo, Cheng; Zhang, Hao
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Drug Discovery & Design Ctr, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Fudan Univ, Sch Pharm, Shanghai, Peoples R China 4.Nanchang Univ, Sch Pharm, Nanchang, Jiangxi, Peoples R China
推荐引用方式 GB/T 7714	Xu, Heng,Tang, Zhengkuan,Zuo, Yu,et al. Molecular dynamics simulation revealed the intrinsic conformational change of cellular inhibitor of apoptosis protein-1[J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,2020,38(4):975-984.
APA	Xu, Heng.,Tang, Zhengkuan.,Zuo, Yu.,Xiong, Fengmin.,Chen, Kaixian.,...&Zhang, Hao.(2020).Molecular dynamics simulation revealed the intrinsic conformational change of cellular inhibitor of apoptosis protein-1.JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,38(4),975-984.
MLA	Xu, Heng,et al."Molecular dynamics simulation revealed the intrinsic conformational change of cellular inhibitor of apoptosis protein-1".JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS 38.4(2020):975-984.