Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide

doi:10.1016/j.ijbiomac.2020.01.305

	Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide
	Li, Meixia 1; Yue, Han 1; Wang, Yeqing 1; Guo, Ciliang 1; Du, Zhenyun 1; Jin, Can 2; Ding, Kan 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2020-04-15
卷号	149 页码:717-723
关键词	Dendrobium officinale Polysaccharide Gut microbiota Metagenomics sequencing SCFAs
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2020.01.305
通讯作者	Ding, Kan(dingkan@simm.ac.cn)
英文摘要	Although immunomodulatory activities of Dendrobium officinale polysaccharide has been investigated for many years, yet the potential contribution of its metabolite derived from intestinal microbes on immunoregulation effect has not been reported. In this study, polysaccharide DOW-5B with average molecular weight of 39.4 kDa was isolated from the stem of Dendrobium officinale Kimura et Migo. The carbohydrate content was 91.97% and no protein was detected. The monosaccharide analysis showed this polysaccharide was composed of glucuronic acid and glucose at a molar ratio (M/G) of 1.2:19.4. Animal test indicated DOW-5B increased the diversity of gut microbiota on mice. Beneficial microbes such as Ruminococcus, Eubacterium, Clostridium, Bifidobacterium, Parabacteroides and Akkermansia muciniphila increased while harmful bacteria in Proteobacteria decreased. Surprisingly, DOW-5B promoted gut microbes to generate more butyrate and mainly produced by Parabacteroides_sp_HGS0025. Further, we found the health of large intestine as well as immunity response of mice was improved. In addition, Parabacteroides_sp_HG50025 positively correlated with butyrate, IgM, IL-10, and INF-alpha products in intestine and mice blood, respectively. The data suggested that Dendrobium officinale polysaccharide has function on immunity may be mediated by butyrate. It adds new evidence to support the basis of how herbal polysaccharides affect immunity. (C) 2020 Elsevier B.V. All rights reserved.
资助项目	New Drug Creation and Manufacturing Program, Ministry of Science and Technology, the People Republic of China[2019ZX09735001] ; National Natural Science Foundation of China[31800673] ; National Natural Science Foundation of China[31670814]
WOS关键词	CHAIN FATTY-ACIDS ; DIETARY FIBER ; BACTERIA ; DIVERSITY ; METABOLISM ; PHYSIOLOGY ; STEM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000525795400071
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281113]
专题	中国科学院上海药物研究所
通讯作者	Ding, Kan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Meixia,Yue, Han,Wang, Yeqing,et al. Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,149:717-723.
APA	Li, Meixia.,Yue, Han.,Wang, Yeqing.,Guo, Ciliang.,Du, Zhenyun.,...&Ding, Kan.(2020).Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,149,717-723.
MLA	Li, Meixia,et al."Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 149(2020):717-723.