Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase

doi:10.1038/s41467-020-15268-y

	Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase
	Gao, Yu 1; Zhang, Hongmei 1; Fan, Minrui 1,3; Jia, Chenjun 1,4; Shi, Lifang 1; Pan, Xiaowei 1; Cao, Peng 1; Zhao, Xuelin 1; Chang, Wenrui 1,2; Li, Mei 1
刊名	NATURE COMMUNICATIONS
	2020-03-23
卷号	11 期号:1 页码:11
ISSN号	2041-1723
DOI	10.1038/s41467-020-15268-y
通讯作者	Li, Mei(meili@ibp.ac.cn)
英文摘要	Long-chain alk(a/e)nes represent the major constituents of conventional transportation fuels. Biosynthesis of alkanes is ubiquitous in many kinds of organisms. Cyanobacteria possess two enzymes, acyl-acyl carrier protein (acyl-ACP) reductase (AAR) and aldehyde-deformylating oxygenase (ADO), which function in a two-step alkane biosynthesis pathway. These two enzymes act in series and possibly form a complex that efficiently converts long chain fatty acyl-ACP/fatty acyl-CoA into hydrocarbon. While the structure of ADO has been previously described, structures of both AAR and AAR-ADO complex have not been solved, preventing deeper understanding of this pathway. Here, we report a ligand-free AAR structure, and three AAR-ADO complex structures in which AARs bind various ligands. Our results reveal the binding pattern of AAR with its substrate/cofactor, and suggest a potential aldehyde-transferring channel from AAR to ADO. Based on our structural and biochemical data, we proposed a model for the complete catalytic cycle of AAR.
资助项目	Strategic Priority Research Program of CAS[XDB27020106] ; Strategic Priority Research Program of CAS[XDB08020302] ; National Key R&D Program of China[2017YFA0503702] ; National Key R&D Program of China[2011CBA00900] ; National Natural Science Foundation of China[31770778] ; National Natural Science Foundation of China[31930064] ; Key Research Program of Frontier Sciences of CAS[QYZDB-SSWSMC005]
WOS关键词	ALDEHYDE DEFORMYLATING OXYGENASE ; ENHANCED PRODUCTION ; BIOSYNTHESIS ; DECARBONYLATION ; HYDROCARBONS ; PATHWAYS ; BIOFUELS ; COENZYME ; BINDING ; ALKANES
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000522193100009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280889]
专题	中国科学院上海药物研究所
通讯作者	Li, Mei
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Stanford Univ, Sch Med, Dept Mol & Cellular Physiol, 279 Campus Dr, Stanford, CA 94305 USA 4.Chinese Acad Sci, CAS Key Lab Receptor Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yu,Zhang, Hongmei,Fan, Minrui,et al. Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase[J]. NATURE COMMUNICATIONS,2020,11(1):11.
APA	Gao, Yu.,Zhang, Hongmei.,Fan, Minrui.,Jia, Chenjun.,Shi, Lifang.,...&Li, Mei.(2020).Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase.NATURE COMMUNICATIONS,11(1),11.
MLA	Gao, Yu,et al."Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase".NATURE COMMUNICATIONS 11.1(2020):11.