A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry

doi:10.1002/rcm.8730

	A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry
	Fu, Huan 1,2; Zhang, Qing-li 2; Huang, Xiao-wu 3; Ma, Zheng-hua 2; Zheng, Xiao-li 2; Li, Shi-lin 2; Duan, Hao-nan 2; Sun, Xiao-chu 1; Lin, Fei-fei 2; Zhao, Li-juan 2
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2020-05-15
卷号	34 期号:9 页码:11
ISSN号	0951-4198
DOI	10.1002/rcm.8730
通讯作者	Teng, Guo-sheng(gshteng@163.com) ; Liu, Jia(jia.liu@simm.ac.cn)
英文摘要	Rationale Short-chain fatty acids (SCFAs) are associated with intestinal microbiota and diseases in humans. SCFAs have a low response in mass spectrometry, and in order to increase sensitivity, reduce sample consumption, shorten analysis time, and simplify sample preparation steps, a derivatization method was developed. Methods We converted seven SCFAs into amide derivatives with 4-aminomethylquinoline. The reaction occurred for 20 min at room temperature. The analytes were separated on a reversed-phase C18 column and quantitated in the positive ion electrospray ionization mode using multiple reaction monitoring. Acetic acid-d(4) was used as the stable-isotope-labeled surrogate analyte for acetic acid in the working solutions, while the other stable-isotope-labeled standards were used as internal standards (ISs). Results Method validation showed that the intra-day and inter-day precision of quantitation for the seven SCFAs over the whole concentration range was <= 3.8% (n = 6). The quantitation accuracy ranged from 85.5% to 104.3% (n = 6). Most important, the collected feces were vortexed immediately with ethanol. Conclusions This study provides a new derivatization method for a precise, accurate, and rapid quantitation of SCFAs in human feces using ultra-performance liquid chromatography/tandem mass spectrometry. This method successfully determined the concentration of SCFAs in human feces and could assist in the exploration of intestinal microbiota and diseases.
资助项目	National Natural Science Foundation of China[81803610] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
WOS关键词	QUANTIFICATION ; METABOLITES ; BIOMARKERS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种	英语
出版者	WILEY
WOS记录号	WOS:000529870500012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280574]
专题	中国科学院上海药物研究所
通讯作者	Teng, Guo-sheng; Liu, Jia
作者单位	1.Changchun Univ Technol, Sch Chem & Life Sci, Changchun 130012, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.PLA Army Gen Hosp, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Fu, Huan,Zhang, Qing-li,Huang, Xiao-wu,et al. A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2020,34(9):11.
APA	Fu, Huan.,Zhang, Qing-li.,Huang, Xiao-wu.,Ma, Zheng-hua.,Zheng, Xiao-li.,...&Liu, Jia.(2020).A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,34(9),11.
MLA	Fu, Huan,et al."A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 34.9(2020):11.