Antibody-free enzyme-assisted chemical approach for detection of N-6-methyladenosine

doi:10.1038/s41589-020-0525-x

	Antibody-free enzyme-assisted chemical approach for detection of N-6-methyladenosine
	Wang, Ye 2; Xiao, Yu 2; Dong, Shunqing 2; Yu, Qiong 2; Jia, Guifang 1,2
刊名	NATURE CHEMICAL BIOLOGY
	2020-04-27
页码	12
ISSN号	1552-4450
DOI	10.1038/s41589-020-0525-x
通讯作者	Jia, Guifang(guifangjia@pku.edu.cn)
英文摘要	The inert chemical property of RNA modification N-6-methyladenosine (m(6)A) makes it very challenging to detect. Most m(6)A sequencing methods rely on m(6)A-antibody immunoprecipitation and cannot distinguish m(6)A and N-6,2 '-O-dimethyladenosine modification at the cap +1 position (cap m(6)A(m)). Although the two antibody-free methods (m(6)A-REF-seq/MAZTER-seq and DART-seq) have been developed recently, they are dependent on m(6)A sequence or cellular transfection. Here, we present an antibody-free, FTO-assisted chemical labeling method termed m(6)A-SEAL for specific m(6)A detection. We applied m(6)A-SEAL to profile m(6)A landscapes in humans and plants, which displayed the known m(6)A distribution features in transcriptome. By doing a comparison with all available m(6)A sequencing methods and specific m(6)A sites validation by SELECT, we demonstrated that m(6)A-SEAL has good sensitivity, specificity and reliability for transcriptome-wide detection of m(6)A. Given its tagging ability and FTO's oxidation property, m(6)A-SEAL enables many applications such as enrichment, imaging and sequencing to drive future functional studies of m(6)A and other modifications.
资助项目	National Basic Research Program of China[2019YFA0802201] ; National Basic Research Program of China[2017YFA0505201] ; National Natural Science Foundation of China[21822702] ; National Natural Science Foundation of China[21820102008] ; National Natural Science Foundation of China[21432002] ; State Key Laboratory of Drug Research
WOS关键词	MESSENGER-RNA ; DNA METHYLATION ; NUCLEAR-RNA ; REVEALS ; MOUSE ; N6-METHYLADENOSINE ; TRANSCRIPTOME ; N-6-ADENINE ; DEMETHYLASE
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000529078300001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280469]
专题	中国科学院上海药物研究所
通讯作者	Jia, Guifang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 2.Peking Univ, Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci,Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ye,Xiao, Yu,Dong, Shunqing,et al. Antibody-free enzyme-assisted chemical approach for detection of N-6-methyladenosine[J]. NATURE CHEMICAL BIOLOGY,2020:12.
APA	Wang, Ye,Xiao, Yu,Dong, Shunqing,Yu, Qiong,&Jia, Guifang.(2020).Antibody-free enzyme-assisted chemical approach for detection of N-6-methyladenosine.NATURE CHEMICAL BIOLOGY,12.
MLA	Wang, Ye,et al."Antibody-free enzyme-assisted chemical approach for detection of N-6-methyladenosine".NATURE CHEMICAL BIOLOGY (2020):12.