SR mu CT Reveals 3D Microstructural Alterations of the Vascular and Neuronal Network in a Rat Model of Chronic Compressive Thoracic Spinal Cord Injury

doi:10.14336/AD.2019.0529

	SR mu CT Reveals 3D Microstructural Alterations of the Vascular and Neuronal Network in a Rat Model of Chronic Compressive Thoracic Spinal Cord Injury
	Jiang, Liyuan 3,4; Cao, Yong 3,4; Liu, Zhen 5; Ni, Shuangfei 3,4; Liu, Jun 5; Ha, Yoon 6; Luo, Zixiang 3,4; Li, Chengjun 3,4; Liu, Shaohua 4; Li, Jingsong 7
刊名	AGING AND DISEASE
	2020-06-01
卷号	11 期号:3 页码:603-617
关键词	chronic spinal cord injury neurovascular unit spinal cord microvasculature SR mu CT 3D
ISSN号	2152-5250
DOI	10.14336/AD.2019.0529
通讯作者	Wu, Tianding(tiandingwu@hotmail.com) ; Hu, Jianzhong(jianzhonghu@hotmail.com)
英文摘要	The complex pathology of chronic thoracic spinal cord compression involves vascular and neuroarchitectural repair processes that are still largely unknown. In this study, we used synchrotron radiation microtomography (SR mu CT) to quantitatively characterize the 3D temporal-spatial changes in the vascular and neuronal network after chronic thoracic spinal cord compression in order to obtain further insights into the pathogenesis of this disease and to elucidate its underlying mechanisms. Direct 3D characterization of the spinal cord microvasculature and neural microstructure of the thoracic spinal cord was successfully reconstructed. The significant reduction in vasculature and degeneration of neurons in the thoracic spinal cord visualized via SR mu CT after chronic compression were consistent with the changes detected by immunofluorescence staining. The 3D morphological measurements revealed significant reductions of neurovascular parameters in the thoracic spinal cord after 1 month of compression and became even worse after 6 months without relief of compression. In addition, the distinct 3D morphological twist and the decrease in branches of the central sulcal artery after chronic compression vividly displayed that these could be the potential triggers leading to blood flow reduction and neural deficits of the thoracic spinal cord. Our findings propose a novel methodology for the 3D analysis of neurovascular repair in chronic spinal cord compression, both qualitatively and quantitatively. The results indicated that compression simultaneously caused vascular dysfunction and neuronal network impairment, which should be acknowledged as concurrent events after chronic thoracic spinal cord injury. Combining neuroprotection with vasoprotection may provide promising therapeutic targets for chronic thoracic spinal cord compression.
资助项目	National Natural Science Foundation of China[81874004] ; National Natural Science Foundation of China[81672174] ; National Natural Science Foundation of China[81301522] ; National Natural Science Foundation of China[81371956] ; National Natural Science Foundation of China[81301542] ; National Natural Science Foundation of China[81502331] ; Fundamental Research Funds for the Central Universities of Central South University[201 8zzts254] ; Science Foundation of Xiangya Hospital for Young Scholar[2017Q1] ; New Xiangya Talent Project of the Third Xiangya Hosipital of Central South University[JY201510] ; Natural Science Foundation of Hunan Province[2016JJ3176] ; Natural Science Foundation of Hunan Province[2019JJ50959]
WOS关键词	NEUROVASCULAR UNIT ; 3-DIMENSIONAL ALTERATION ; EPIDEMIOLOGY ; OSSIFICATION ; TOMOGRAPHY ; LIGAMENT
WOS研究方向	Geriatrics & Gerontology
语种	英语
出版者	INT SOC AGING & DISEASE
WOS记录号	WOS:000533551200014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280268]
专题	中国科学院上海药物研究所
通讯作者	Wu, Tianding; Hu, Jianzhong
作者单位	1.Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Cent South Univ, Xiangya Hosp, Res Ctr Sports Med, Dept Sports Med, Changsha, Peoples R China 3.Key Lab Organ Injury Aging & Regenerat Med Hunan, Changsha, Peoples R China 4.Cent South Univ, Xiangya Hosp, Dept Spine Surg, Changsha, Peoples R China 5.First Chenzhou Peoples Hosp, Chenzhou, Peoples R China 6.Yonsei Univ, Spine & Spinal Cord Inst, Dept Neurosurg, Coll Med, Seoul, South Korea 7.Cent South Univ, Xiangya Hosp 3, Depaihnent Spine Surg, Changsha, South Korea
推荐引用方式 GB/T 7714	Jiang, Liyuan,Cao, Yong,Liu, Zhen,et al. SR mu CT Reveals 3D Microstructural Alterations of the Vascular and Neuronal Network in a Rat Model of Chronic Compressive Thoracic Spinal Cord Injury[J]. AGING AND DISEASE,2020,11(3):603-617.
APA	Jiang, Liyuan.,Cao, Yong.,Liu, Zhen.,Ni, Shuangfei.,Liu, Jun.,...&Hu, Jianzhong.(2020).SR mu CT Reveals 3D Microstructural Alterations of the Vascular and Neuronal Network in a Rat Model of Chronic Compressive Thoracic Spinal Cord Injury.AGING AND DISEASE,11(3),603-617.
MLA	Jiang, Liyuan,et al."SR mu CT Reveals 3D Microstructural Alterations of the Vascular and Neuronal Network in a Rat Model of Chronic Compressive Thoracic Spinal Cord Injury".AGING AND DISEASE 11.3(2020):603-617.