Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism

doi:10.3390/cells9020391

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	Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism
	Zhang, Zhichao 1; Zhang, Qing 1; Guan, Shaohua 1; Shi, Hualin 1
刊名	CELLS
	2020
卷号	9 期号:2 页码:391
关键词	HAEMOPHILUS-INFLUENZAE RD GRAM-NEGATIVE BACTERIA ESCHERICHIA-COLI OUTER-MEMBRANE FATTY-ACID GUANOSINE TETRAPHOSPHATE CARDIOLIPIN SYNTHASE ADAPTIVE EVOLUTION ENVELOPE MODEL
DOI	10.3390/cells9020391
英文摘要	The processes involved in cell growth are extremely complicated even for a single cell organism such as Escherichia coli, while the relationship between growth rate and cell size is simple. We aimed to reveal the systematic link between them from the aspect of the genome-scale metabolic network. Since the growth rate reflects metabolic rates of bacteria and the cell size relates to phospholipid synthesis, a part of bacterial metabolic networks, we calculated the cell length from the cardiolipin synthesis rate, where the cardiolipin synthesis reaction is able to represent the phospholipid metabolism of Escherichia coli in the exponential growth phase. Combined with the flux balance analysis, it enables us to predict cell length and to examine the quantitative relationship between cell length and growth rate. By simulating bacteria growing in various nutrient media with the flux balance analysis and calculating the corresponding cell length, we found that the increase of the synthesis rate of phospholipid, the cell width, and the protein fraction in membranes caused the increase of cell length with growth rate. Different tendencies of phospholipid synthesis rate changing with growth rate result in different relationships between cell length and growth rate. The effects of gene deletions on cell size and growth rate are also examined. Knocking out the genes, such as Delta tktA, Delta tktB, Delta yqaB, Delta pgm, and Delta cysQ, affects growth rate largely while affecting cell length slightly. Results of this method are in good agreement with experiments.
学科主题	Cell Biology
语种	英语
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/27074]
专题	理论物理研究所_理论物理所1978-2010年知识产出
作者单位	1.Univ Chinese Acad Sci, Sch Phys Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Theoret Phys, CAS Key Lab Theoret Phys, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zhichao,Zhang, Qing,Guan, Shaohua,et al. Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism[J]. CELLS,2020,9(2):391.
APA	Zhang, Zhichao,Zhang, Qing,Guan, Shaohua,&Shi, Hualin.(2020).Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism.CELLS,9(2),391.
MLA	Zhang, Zhichao,et al."Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism".CELLS 9.2(2020):391.