Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study

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	Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study
	Zhang Chao 3; Wang Lu 2; Guan Xin 3; Fawcett J Paul 1; Zhao Limei 2; Sun Yantong 3; Gu Jingkai 3
刊名	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
	2010
卷号	26 期号:6 页码:910-914
关键词	FAMOTIDINE HPLC BIOEQUIVALENCE METABOLITES NIZATIDINE VOLUNTEERS ASSAY Ranitidine Pharmacokinetics Human plasma Protein precipitation LC-MS/MS
ISSN号	1005-9040
其他题名	Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study
英文摘要	A rapid and sensitive assay based on high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of ranitidine(RAN) in human plasma with codeine as internal standard(IS). After protein precipitation with acetonitrile, the analyte and IS were separated on a Zorbax SB-Aq C-18 column(150 mmx4.6 mm i. d., 5 mu m) eluted with a mobile phase consisting of methanol/acetonitrile/10 mmol/L ammonium acetate containing 1% formic acid(pH=2.4)(volume ratio 12.5:12.5:75) at a flow rate of 1.0 mL/min. Detection was performed by electrospray ionization in the positive ion mode followed by the multiple reaction monitoring(MRM) of the transitions of RAN at m/z 315.1 -> 176.3 and of IS at m/z 300.1 -> 165.1. The method was linear over a concentration range of 1-1000 ng/mL(r=0.9991) with a lower limit of quantitation(LLOQ) of 1 ng/mL and a limit of detection(LOD) of 0.3 ng/mL. Accuracy as relative error was from -0.01% to -1.7% and intra-day and inter-day precisions as relative standard deviation were <= 8.9% and <= 5.5%, respectively. The method was successfully applied to a pharmacokinetic study of ranitidine, getting a single oral dose(160 mg) to healthy volunteers.
资助项目	[National High-Tech Research and Development Program of China] ; [National Natural Science Foundation of China]
语种	英语
CSCD记录号	CSCD:4093582
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/154155]
专题	金属研究所_中国科学院金属研究所
作者单位	1.University Otago, Sch Pharm, Dunedin, New Zealand 2.中国科学院金属研究所 3.吉林大学
推荐引用方式 GB/T 7714	Zhang Chao,Wang Lu,Guan Xin,et al. Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2010,26(6):910-914.
APA	Zhang Chao.,Wang Lu.,Guan Xin.,Fawcett J Paul.,Zhao Limei.,...&Gu Jingkai.(2010).Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,26(6),910-914.
MLA	Zhang Chao,et al."Rapid Determination of Ranitidine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Clinical Pharmacokinetic Study".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 26.6(2010):910-914.