In vitro insights into the role of copper ions released from selective laser melted CoCrW-xCu alloys in the potential attenuation of inflammation and osteoclastogenesis

doi:10.1016/j.jmst.2019.09.016

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	In vitro insights into the role of copper ions released from selective laser melted CoCrW-xCu alloys in the potential attenuation of inflammation and osteoclastogenesis
	Lu, Yanjin 1,4; Xu, Xiongcheng 3; Yang, Chunguang 2; Ren, Ling 2; Luo, Kai 3; Yang, Ke 2; Lin, Jinxin 1,4
刊名	JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
	2020-03-15
卷号	41 页码:56-67
关键词	Osteoclast Selective laser melting Macrophage Cu-bearing biomedical alloy Biocompatibility
ISSN号	1005-0302
DOI	10.1016/j.jmst.2019.09.016
通讯作者	Yang, Chunguang(cgyang@imr.ac.cn) ; Luo, Kai(luokai39@163.com) ; Lin, Jinxin(franklin@fjirsm.ac.cn)
英文摘要	In this study, the CoCrW-xCu alloys (x= 2, 3 and 4 wt%) were fabricated using selective laser melting (abbreviated as Co-2Cu, Co-3Cu and Co-4Cu) with the purpose of reducing the inflammation responses and the activity of osteoclast. The metal ions releasing test showed that when the Cu content was less than 4 wt%, the releasing amount of Co and Cr ions was very small; however, when 4 wt% Cu was added in the CoCrW based alloy, the quantity of Co ions was significantly elevated with respect to the other groups due to the segregation of precipitates in the matrix; the Cu2+ ion quantity of the Co-2Cu, Co-3Cu and Co-4Cu alloys were 0.05, 0.09 and 0.27 mu g/(L cm(2)) after 7 d immersion, respectively; the RT-qPCR and ELISA data indicated that the expression levels of the pro-inflammatory cytokines (TNF-alpha and IL-6) were down-regulated in the Co-3Cu and Co-4Cu groups, whereas the expression level of the anti-inflammatory cytokine (IL-10) was up-regulated in all CoCrW-xCu alloys; meanwhile, the Cu-containing CoCrW alloys significantly down-regulated the expression of the NF-kappa B signal pathway in a Cu content-dependent manner, and the downstream transcription factors of NF-kappa B signal pathway including NFATc1, TRAP and Cath-K were also down-regulated via potentially manipulating the NF-kappa B signal pathway. After comprehensive consideration, it is considered that the Co-3Cu alloy is a potential material for self-alleviating inflammatory responses. (C) 2019 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.
资助项目	National Natural Science Foundation of China[51801198] ; National Key RD Plan[2016YFC1100502]
WOS研究方向	Materials Science ; Metallurgy & Metallurgical Engineering
语种	英语
出版者	JOURNAL MATER SCI TECHNOL
WOS记录号	WOS:000513986900006
资助机构	National Natural Science Foundation of China ; National Key RD Plan
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/137488]
专题	金属研究所_中国科学院金属研究所
通讯作者	Yang, Chunguang; Luo, Kai; Lin, Jinxin
作者单位	1.Chinese Acad Sci, Fujian Inst Res Struct Matter, Key Lab Optoelect Mat Chem & Phys, Fuzhou 350001, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 3.Fujian Med Univ, Key Lab Stomatol, Fuzhou 350001, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lu, Yanjin,Xu, Xiongcheng,Yang, Chunguang,et al. In vitro insights into the role of copper ions released from selective laser melted CoCrW-xCu alloys in the potential attenuation of inflammation and osteoclastogenesis[J]. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,2020,41:56-67.
APA	Lu, Yanjin.,Xu, Xiongcheng.,Yang, Chunguang.,Ren, Ling.,Luo, Kai.,...&Lin, Jinxin.(2020).In vitro insights into the role of copper ions released from selective laser melted CoCrW-xCu alloys in the potential attenuation of inflammation and osteoclastogenesis.JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,41,56-67.
MLA	Lu, Yanjin,et al."In vitro insights into the role of copper ions released from selective laser melted CoCrW-xCu alloys in the potential attenuation of inflammation and osteoclastogenesis".JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY 41(2020):56-67.