Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes

doi:10.1016/j.carbpol.2018.10.067

CORC > 金属研究所 > 中国科学院金属研究所

	Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes
	Lv, Xiaojie 1,2; Liu, Yunen 3; Song, Subin 2; Tong, Changci 3; Shi, Xiuyun 3; Zhao, Yan 2; Zhang, Jinsong 2; Hou, Mingxiao 3
刊名	CARBOHYDRATE POLYMERS
	2019-02-01
卷号	205 页码:312-321
关键词	Injectable hydrogels Chitosan oligosaccharide Electrostatic interactions Storage modulus Wound healing
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2018.10.067
通讯作者	Zhao, Yan(yanzhao@imr.ac.cn) ; Zhang, Jinsong(jshzhang@imr.ac.cn)
英文摘要	In situ injectable hydrogels for wound healing based on carboxymethyl chitosan (CMCS) and alginate were developed in this work. The liquid mixture of CMCS and alginate solutions formed a gel by polyelectrolyte complexation after addition of D-glucono-delta-lactone (GDL), which slowly hydrolyzed and donated protons. When chitosan oligosaccharide (COS) was added into the mixture, a two-stage gelling process occurred. The primary gelling process was similar to that of the hydrogel without COS, while the secondary gelling process appeared about 20 min later, and much stronger hydrogels with storage modulus G' about 1 MPa, 10(4) times higher, were obtained. COS also significantly influenced the microstructure of hydrogels as well as their biological activities. The hydrogels with 0.5% of COS significantly promoted proliferation of human umbilical cord mesenchymal stem cells (HUMSCs). These injectable hydrogels, especially when COS was added, remarkably accelerated the wound healing process in a mouse skin defect model. Microscopic wound analysis showed an increase of the thickness and integrity of epidermal tissue, increased formation of collagen fibers, and enhanced expression of vascular endothelial growth factor as compared to the control group.
资助项目	National Key Research and Development Plan[2016YFB0301502] ; National Natural Science Foundation of China[U1608255]
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000450093200036
资助机构	National Key Research and Development Plan ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/130446]
专题	金属研究所_中国科学院金属研究所
通讯作者	Zhao, Yan; Zhang, Jinsong
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Peoples R China 3.Gen Hosp Shenyang Mil Command, Dept Emergency Med, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Lv, Xiaojie,Liu, Yunen,Song, Subin,et al. Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes[J]. CARBOHYDRATE POLYMERS,2019,205:312-321.
APA	Lv, Xiaojie.,Liu, Yunen.,Song, Subin.,Tong, Changci.,Shi, Xiuyun.,...&Hou, Mingxiao.(2019).Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes.CARBOHYDRATE POLYMERS,205,312-321.
MLA	Lv, Xiaojie,et al."Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes".CARBOHYDRATE POLYMERS 205(2019):312-321.