A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-) | |
Liu Junchi1,2,4; Liu Weiguo2,3; Hu Jing2 | |
刊名 | PEDOSPHERE |
2018-12-01 | |
卷号 | 28期号:6页码:943-951 |
关键词 | air-drying freezing ion exchange isotopic fractionation nitrogen isotope tracing soil extraction soil storage |
ISSN号 | 1002-0160 |
DOI | 10.1016/S1002-0160(17)60412-0 |
通讯作者 | Liu Weiguo(liuwg@loess.llqg.ac.cn) |
英文摘要 | Nitrogen (N) isotope ratio (delta N-15) of soil extractable NO(3)(-)plays a pivotal role in the study of N biogeochemical circulation in ecosystems. However, the NO(3)(-)concentration and its isotope composition of soil samples are unstable, making sample storage critical for preserving the N isotope composition of extracted soil Nevertheless, studies on the appropriate selection of storage methods after soil sampling are scarce. In this study, we compared two commonly used methods for storing soil samples and investigated the stability of N isotopes of soil The results demonstrated that no significant changes in the NO(3)(-)concentration and delta N-15 value occurred in the samples stored at -18 degrees C. However, the soil NO(3)(-)concentration markedly increased, and NO3- delta N-15 value significantly changed after air-drying storage. Meanwhile, we also found that NO3- and its delta N-15 were well preserved in the filtered soil extracts after 1 month. In contrast, the NO(3)(-)concentration gradually decreased and the N-15 in NO(3)(-)was gradually enriched in the bactericidal agent-containing soil mixture solution during the storage period. Overall, our results indicated that N isotopes of NO3- could be effectively preserved in frozen-stored soil samples or filtered soil extracts. For field investigations conducted in remote areas and continued for a long-time period (and lacking a refrigerant supply), soil extraction/filtration using a CaSO4-saturated solution may be a superior preparation and storage method for analyzing N isotopes of soil NO3-. |
资助项目 | Key Deployment Project from Chinese Academy of Sciences[KZZD-EW-04-06] ; National Natural Science Foundation of China[41373022] ; National Basic Research Program of China[2013CB955900] |
WOS关键词 | SHORT-TERM CHANGES ; MICROBIAL BIOMASS ; NATURAL-ABUNDANCE ; FOREST ; NITRATE ; PLANT ; NITRIFICATION ; STORAGE ; FRACTIONATION ; DELTA-N-15 |
WOS研究方向 | Agriculture |
语种 | 英语 |
出版者 | SCIENCE PRESS |
WOS记录号 | WOS:000456949800010 |
资助机构 | Key Deployment Project from Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Basic Research Program of China |
内容类型 | 期刊论文 |
源URL | [http://ir.ieecas.cn/handle/361006/14437] |
专题 | 地球环境研究所_加速器质谱中心 |
通讯作者 | Liu Weiguo |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Earth Environm, State Key Lab Loess & Quaternary Geol, Xian 710075, Shaanxi, Peoples R China 3.Xi An Jiao Tong Univ, Sch Human Settlement & Civil Engn, Xian 710049, Peoples R China 4.Chinese Acad Sci, Key Lab Vertebrate Evolut & Human Origin, Inst Vertebrate Paleontol & Paleoanthropol, Beijing 100044, Peoples R China |
推荐引用方式 GB/T 7714 | Liu Junchi,Liu Weiguo,Hu Jing. A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-)[J]. PEDOSPHERE,2018,28(6):943-951. |
APA | Liu Junchi,Liu Weiguo,&Hu Jing.(2018).A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-).PEDOSPHERE,28(6),943-951. |
MLA | Liu Junchi,et al."A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-)".PEDOSPHERE 28.6(2018):943-951. |
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