Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples
	Liu, Lanhua ; Zhou, Xiaohong ; Lu, Yun ; Shi, Hanchang ; Ma, Mei ; Yu, Tong
刊名	ENVIRONMENT INTERNATIONAL
	2019-11-01
卷号	132 页码:-
关键词	Estrogen receptor Small-molecule-protein conjugate Optical fiber biosensor 17 beta-estradiol equivalent concentration Wastewater sample
ISSN号	0160-4120
英文摘要	Establishing biosensors to map a comprehensive picture of potential estrogen-active chemicals remains challenging and must be addressed. Herein, we describe an estrogen receptor (ER)-based evanescent wave fluorescent biosensor by using a triple functional small-molecule-protein conjugate as a signal probe for the determination of estrogenic activities in water samples. The signal probe, consisting of a Cy5.5-labelled streptavidin (STV) moiety and a 17 beta-estradiol (E-2) moiety, acts simultaneously as signal conversion, signal recognition and signal report elements. When xenoestrogens compete with the E-2 moiety of conjugate in binding to the ER, the unbound conjugates are released, and their STV moiety binds with desthiobiotin (DTB) modified on the optical fiber via the STV-DTB affinity interactions. Signal probe detection is accomplished by fluorescence emission induced by an evanescent field, which positively relates with the estrogenic activities in samples. Quantification of estrogenic activity expressed as E-2 equivalent concentration (EEQ) can be achieved with a detection limit of 1.05 mu g/L EEQ by using three times standard deviation of the mean blank values and a linear calibration range from 20.8 to 476.7 mu g/L EEQ. The optical fiber system is robust enough for hundreds of sensing cycles. The biosensor-based determination of estrogenic activities in wastewater samples obtained from a full-scale wastewater treatment plant is consistent with that measured by the two-hybrid recombinant yeast bioassay.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43544]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
作者单位	1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 3.Tsinghua Univ, Sch Environm, State Key Joint Lab ESPC, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lanhua,Zhou, Xiaohong,Lu, Yun,et al. Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples[J]. ENVIRONMENT INTERNATIONAL,2019,132:-.
APA	Liu, Lanhua,Zhou, Xiaohong,Lu, Yun,Shi, Hanchang,Ma, Mei,&Yu, Tong.(2019).Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples.ENVIRONMENT INTERNATIONAL,132,-.
MLA	Liu, Lanhua,et al."Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples".ENVIRONMENT INTERNATIONAL 132(2019):-.