One-year survey of opportunistic premise plumbing pathogens and free-living amoebae in the tap-water of one northern city of China

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	One-year survey of opportunistic premise plumbing pathogens and free-living amoebae in the tap-water of one northern city of China
	Liu, Lizhong ; Xing, Xueci ; Hu, Chun ; Wang, Haibo
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2019-03-01
卷号	77 页码:20-31
关键词	Opportunistic premise plumbing pathogens (OPPPs) Free-living amoebae (FLA) Drinking water distribution systems (DWDS) Tap-water Quantitative PCR (qPCR)
ISSN号	1001-0742
英文摘要	In this study, qPCR was used to quantify opportunistic premise plumbing pathogens (OPPPs) and free-living amoebae in 11 tap water samples collected over four seasons from a city in northern China. Results demonstrated that the average numbers of gene copies of Legionella spp. and Mycobacterium spp. were significantly higher than those of Aeromonas spp. (p < 0.05). Legionelia spp. and Mycobacterium spp. were 100% (44/44) positively detected while P. aeruginosa and Aeromonas spp. were 79.54% (35/44) and 77.27% (34/44) positively detected. Legionella pneumophila was only detected in 4 samples (4/44), demonstrating its occasional occurrence. No Mycobacterium avium or Naegleria fowleri was detected in any of the samples. The average gene copy numbers of target OPPPs were the highest in summer, suggesting seasonal prevalence of OPPPs. Average gene copy numbers of OPPPs in the taps of low-use-frequency were higher than in taps of high-use-frequency, but the difference was not significant for some OPPPs (p > 0.05). Moderate negative correlations between the chlorine concentration and the gene copy numbers of OPPPs were observed by Spearman analysis (r s ranged from -0.311 to -0.710, p < 0.05). However, no significant correlations existed between OPPPs and AOC, BDOC, or turbidity. Moderate positive correlations were observed between the target microorganisms, especially for Acanthamoeba spp., through Spearman analysis (p < 0.05). Based on our studies, it is proposed that disinfectant concentration, season, taps with different-use frequency, OPPP species, and potential microbial correlations should be considered for control of OPPPs in tap water. (C)2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43499]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Drinking Water Sci & Technol, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China 2.Guangzhou Univ, Key Lab Water Qual & Conservat Pearl River Delta, Minist Educ, Inst Environm Res Greater Bay, Guangzhou 510006, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lizhong,Xing, Xueci,Hu, Chun,et al. One-year survey of opportunistic premise plumbing pathogens and free-living amoebae in the tap-water of one northern city of China[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2019,77:20-31.
APA	Liu, Lizhong,Xing, Xueci,Hu, Chun,&Wang, Haibo.(2019).One-year survey of opportunistic premise plumbing pathogens and free-living amoebae in the tap-water of one northern city of China.JOURNAL OF ENVIRONMENTAL SCIENCES,77,20-31.
MLA	Liu, Lizhong,et al."One-year survey of opportunistic premise plumbing pathogens and free-living amoebae in the tap-water of one northern city of China".JOURNAL OF ENVIRONMENTAL SCIENCES 77(2019):20-31.