Myclobutanil accumulation, transcriptional alteration, and tissue injury in lizards (Eremias argus) treated with myclobutanil enantiomers

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Myclobutanil accumulation, transcriptional alteration, and tissue injury in lizards (Eremias argus) treated with myclobutanil enantiomers
	Hao, Weiyu ; Zhang, Yanfeng ; Xie, Yun ; Guo, Baoyuan ; Chang, Jing ; Li, Jianzhong ; Xu, Peng ; Wang, Huili
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2019-04-30
卷号	171 页码:247-255
关键词	Myclobutanil Lizards Enantioselective Bioaccumulation Gene expression
ISSN号	0147-6513
英文摘要	Enantioselective toxicokinetics, accumulation, and toxicity of myclobutanil were investigated by oral exposure of myclobutanil enantiomers to lizards. After a single oral administration, the absorption half-lives (t(1/2Ka)) and elimination half-lives (t(1/2K)) were in the range of 0.133-14.828 and 3.641-17.682 h, respectively. The absorption and elimination half-lives of (+)-myclobutanil showed no significant differences from those of (-)-myclobutanil in lizard blood, whereas preferential enrichment of (-)-enantiomer was observed in the liver, fat, skin, intestine, lung and kidney. In the bioaccumulation experiments, the residue of (-)-myclobutanil was detected in most tissues at 7, 14, and 28 days, while (+)-myclobutanil was found only in lizard skin, at a concentration lower than that of (-)-myclobutanil. Thus, (-)-myclobutanil was preferentially accumulated in lizards. The transcriptional responses of metabolic enzyme genes indicated that cytochrome P450 1a1 (cyp1a1), cyp2d3, cyp2d6, cyp3a4 and cyp3a7 played a crucial role in the metabolism of (+)-myclobutanil, whereas cyp1a1, cyp2d3, cyp2d6, cyp2c8, and cyp3a4 contributed to the metabolism of (-)-myclobutanil. The difference in metabolism pathways may be a reason for the enantioselectivity of myclobutanil in lizard. Myclobutanil also affected the expression of antioxidant enzyme genes, and the (+)-myclobutanil treatment might produce higher oxidative stress in lizard liver when compared with its antipode. Hepatic histopathological changes such as hepatocellular hypertrophy, nuclear pyknosis, vacuolation, and non-zonal macrovesicular lipid accumulation were observed in the liver of lizards for both (+)-myclobutanil and (-)-myclobutanil treatments. Thus, myclobutanil could affect lizard liver upon multiple exposure. The findings of this study provide specific insights into the enantioselective metabolism and toxicity of chiral triazole fungicides in lizards.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43392]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Shuangqing RD 18, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Yuquan RD 19 A, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hao, Weiyu,Zhang, Yanfeng,Xie, Yun,et al. Myclobutanil accumulation, transcriptional alteration, and tissue injury in lizards (Eremias argus) treated with myclobutanil enantiomers[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2019,171:247-255.
APA	Hao, Weiyu.,Zhang, Yanfeng.,Xie, Yun.,Guo, Baoyuan.,Chang, Jing.,...&Wang, Huili.(2019).Myclobutanil accumulation, transcriptional alteration, and tissue injury in lizards (Eremias argus) treated with myclobutanil enantiomers.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,171,247-255.
MLA	Hao, Weiyu,et al."Myclobutanil accumulation, transcriptional alteration, and tissue injury in lizards (Eremias argus) treated with myclobutanil enantiomers".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 171(2019):247-255.