Electro-driven methanogenic microbial community diversity and variability in the electron abundant niche

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Electro-driven methanogenic microbial community diversity and variability in the electron abundant niche
	Cai, Weiwei ; Liu, Wenzong ; Zhang, Zhaojing ; Feng, Kai ; Ren, Ge ; Pu, Chuanliang ; Li, Jiaqi ; Deng, Ye ; Wang, Aijie
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019-04-15
卷号	661 页码:178-186
关键词	Methanogenic community Basophilic methanogens mcrA
ISSN号	0048-9697
英文摘要	The underlying dynamics of microbial (bacteria and archaea) communities ecologically responding to an applied potential are critical to achieving the goal of enhancing bioenergy recovery but are not sufficiently understood. We built a MEC-AD mode that increased methane production rate by several times (max. 3.8 times) during the startup period compared to control AD, changed the absence or presence of external voltage to provide the pre-, dur-, and post-samples for microbial analysis. From a time and spatially dependent community analysis of electrode-respiring bacteria and methanogens, the corresponding Geobacter developed under the influence of external voltage, pairing with methanogens in the anodic and cathodic biofilm to generate methane. Additionally, at the cathode, the Alkaliphilus (basophilic bacteria) also correspondingly shifted alongside the change of external voltage. The mcrA sequencing confirmed a change in the dominant microbe from acetoclastic (mostly Methanosarcina mazei LYC) to hydrogenotrophic methanogens (mostly basophilic Methanobacterium alcaliphilum) at the cathode with 0.8 V voltage. Overall, the external voltage not only enriched the functional microbes including electrogens and methanogens but also indirectly shifted the composition of the bacterial and archaeal community via disturbing the pH condition. The predictive functional profiling indicated that the cathodic methanogenesis principally followed the metabolism pathway of the hydrogenotrophic methanogens, suggesting the F420 co-enzyme could be the key mediate for electron transfer. All data suggested that the electric stimulation would change and maintain the micro-environmental conditions to shift the bacterial/archaeal community. (C) 2019 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43359]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
作者单位	1.HIT, SKLUWRE, Harbin 150090, Heilongjiang, Peoples R China 2.Dalian Univ Technol, Sch Environm Sci & Technol, Key Lab Ind Ecol & Environm Engn, State Key Lab Fine Chem,Minist Educ, Dalian 116024, Peoples R China 3.Beijing Jiaotong Univ, Sch Civil Engn, Beijing 100044, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Biotechnol, Beijing 100085, Peoples R China
推荐引用方式 GB/T 7714	Cai, Weiwei,Liu, Wenzong,Zhang, Zhaojing,et al. Electro-driven methanogenic microbial community diversity and variability in the electron abundant niche[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,661:178-186.
APA	Cai, Weiwei.,Liu, Wenzong.,Zhang, Zhaojing.,Feng, Kai.,Ren, Ge.,...&Wang, Aijie.(2019).Electro-driven methanogenic microbial community diversity and variability in the electron abundant niche.SCIENCE OF THE TOTAL ENVIRONMENT,661,178-186.
MLA	Cai, Weiwei,et al."Electro-driven methanogenic microbial community diversity and variability in the electron abundant niche".SCIENCE OF THE TOTAL ENVIRONMENT 661(2019):178-186.