C60 Fullerols Enhance Copper Toxicity and Alter the Leaf Metabolite and Protein Profile in Cucumber

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	C60 Fullerols Enhance Copper Toxicity and Alter the Leaf Metabolite and Protein Profile in Cucumber
	Zhao, Lijuan ; Zhang, Huiling ; Wang, Jingjing ; Tian, Liyan ; Li, Fangfang ; Liu, Sijin ; Peralta-Videa, Jose R.; Gardea-Torresdey, Jorge L.; White, Jason C.; Huang, Yuxiong
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2019-02-19
卷号	53 期号:4 页码:2171-2180
ISSN号	0013-936X
英文摘要	Abiotic and biotic stress induce the production of reactive oxygen species (ROS), which limit crop production. Little is known about ROS reduction through the application of exogenous scavengers. In this study, C60 fullerol, a free radical scavenger, was foliar applied to three-week-old cucumber plants (1 or 2 mg/plant) before exposure to copper ions (5 mg/plant). Results showed that C60 fullerols augmented Cu toxicity by increasing the influx of Cu ions into cells (170% and 511%, respectively, for 1 and 2 mg of C60 fullerols/plant). We further use metabolomics and proteomics to investigate the mechanism of plant response to C60 fullerols. Metabolomics revealed that C60 fullerols up-regulated antioxidant metabolites including 3-hydroxyflavone, 1,2,4-benzenetriol, and methyl trans-cinnamate, among others, while it down-regulated cell membrane metabolites (linolenic and palmitoleic acid). Proteomics analysis revealed that C60 fullerols up-regulated chloroplast proteins involved in water photolysis (PSII protein), light-harvesting (CAB), ATP production (ATP synthase), pigment fixation (Mg-PPIX), and electron transport (Cyt b6f). Chlorophyll fluorescence measurement showed that C60 fullerols significantly accelerated the electron transport rate in leaves (13.3% and 9.4%, respectively, for 1 and 2 mg C60 fullerols/plant). The global view of the metabolic pathway network suggests that C60 fullerols accelerated electron transport rate, which induced ROS overproduction in chloroplast thylakoids. Plant activated antioxidant and defense pathways to protect the cell from ROS damaging. The revealed benefit (enhance electron transport) and risk (alter membrane composition) suggest a cautious use of C60 fullerols for agricultural application.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42911]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.CAES, Dept Analyt Chem, New Haven, CT 06504 USA 2.Huazhong Univ Sci & Technol, Sch Mat Sci & Engn, 1037 Luoyu Rd, Wuhan 430074, Hubei, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 4.Univ Texas El Paso, Chem Dept, 500 West Univ Ave, El Paso, TX 79968 USA 5.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lijuan,Zhang, Huiling,Wang, Jingjing,et al. C60 Fullerols Enhance Copper Toxicity and Alter the Leaf Metabolite and Protein Profile in Cucumber[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2019,53(4):2171-2180.
APA	Zhao, Lijuan.,Zhang, Huiling.,Wang, Jingjing.,Tian, Liyan.,Li, Fangfang.,...&Ji, Rong.(2019).C60 Fullerols Enhance Copper Toxicity and Alter the Leaf Metabolite and Protein Profile in Cucumber.ENVIRONMENTAL SCIENCE & TECHNOLOGY,53(4),2171-2180.
MLA	Zhao, Lijuan,et al."C60 Fullerols Enhance Copper Toxicity and Alter the Leaf Metabolite and Protein Profile in Cucumber".ENVIRONMENTAL SCIENCE & TECHNOLOGY 53.4(2019):2171-2180.