Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher
	Wang, Chao 2; Li, Yapiao 2; Zhou, Ce ; Zhao, Qiang 2
刊名	MICROCHIMICA ACTA
	2019-11-01
卷号	186 期号:11 页码:-
关键词	Fluorescent probe Mycotoxin Food safety Envrionmental analysis Fluorophore Nanomaterials Fluorescence quenching Nanoprobe
ISSN号	0026-3672
英文摘要	A fluorometric aptamer based assay is described for rapid and sensitive detection of aflatoxin B1 (AFB1). It is making use of a fluorescein (FAM) labeled anti-AFB1 aptamer and complementary DNA-modified gold nanoparticles (GNPs). In the absence of AFB1, the FAM-labeled aptamers hybridize with complementary DNA strands that were covalently immobilized on GNPs. This results in quenching of the green fluorescence (with excitation/emission peaks at 485/525 nm). In the presence of AFB1, the aptamer probe binds AFB1 and is released from the GNPs. Hence, fluorescence is restored. Under optimized conditions, AFB1 in the concentration range from 61 pM to 4.0 mu M can be detected, and the detection limit is 61 pM. This assay is highly selective for AFB1. It was applied to the determination of AFB1 spiked into 50-fold diluted wine and 20-fold diluted beer.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42544]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chao,Li, Yapiao,Zhou, Ce,et al. Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher[J]. MICROCHIMICA ACTA,2019,186(11):-.
APA	Wang, Chao,Li, Yapiao,Zhou, Ce,&Zhao, Qiang.(2019).Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher.MICROCHIMICA ACTA,186(11),-.
MLA	Wang, Chao,et al."Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher".MICROCHIMICA ACTA 186.11(2019):-.