Butylated hydroxyanisole isomers induce distinct adipogenesis in 3T3-L1 cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Butylated hydroxyanisole isomers induce distinct adipogenesis in 3T3-L1 cells
	Sun, Zhendong ; Yang, Xiaoxi ; Liu, Qian S.; Li, Chuanhai 1; Zhou, Qunfang 1,2; Fiedler, Heidelore 3,4; Liao, Chunyang 1; Zhang, Jianqing 5; Jiang, Guibin 1
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2019-11-05
卷号	379 页码:-
关键词	Butylated hydroxyanisole (BHA) isomers 3T3-L1 cells Adipogenesis Lipogenesis Obesity
ISSN号	0304-3894
英文摘要	Butylated hydroxyanisole (BHA) isomers, as the widely used anthropogenic antioxidants in food, have been revealed to induce endocrine disrupting effects, while the mechanism how BHA isomers regulate the lipogenic differentiation remains to be elucidated. Using 3T3-L1 differentiation model, the effects of BHA isomers, including 2-tert-butyl-4-hydroxyanisole (2-BHA), 3-tert-butyl-4-hydroxyanisole (3-BHA) and their mixture (BHA), on adipogenesis were tested. The results showed that 3-BHA and BHA promoted adipocyte differentiation and enhanced the cellular lipid accumulation through the regulation of the transcriptional and protein levels of the adipogenetic biomarkers, while 2-BHA had no effect. The effective window for 3-BHA induced lipogenesis was the first four days during 3T3-L1 differentiation. BHA isomers showed no binding affinities for peroxisome proliferator activated receptor gamma (PPAR gamma). Instead, the upstream of PPAR gamma signaling pathway, i.e. the phosphorylation of cAMP-response element binding protein (CREB), upregulation of CAAT/enhancer-binding proteins beta (C/EBP beta) and elevated cell proliferation during postconfluent mitosis stage were induced by 3-BHA exposure. Altogether, this study revealed the adipogenic effect of 3-BHA through interference with the upstream events of the PPAR gamma signaling pathway. The authorized usage of BHA as food additives and its occurrence in human sera can potentially contribute to the incidence of obesity, which is of high concern.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42517]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China 4.Orebro Univ, Sch Sci & Technol, MTM Res Ctr, SE-70182 Orebro, Sweden 5.UN Environm MEP, Chem Branch, CH-1219 Chatelaine Ge, Switzerland 6.Shenzhen Ctr Dis Control & Prevent, Dept POPs Lab, Shenzhen 518055, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhendong,Yang, Xiaoxi,Liu, Qian S.,et al. Butylated hydroxyanisole isomers induce distinct adipogenesis in 3T3-L1 cells[J]. JOURNAL OF HAZARDOUS MATERIALS,2019,379:-.
APA	Sun, Zhendong.,Yang, Xiaoxi.,Liu, Qian S..,Li, Chuanhai.,Zhou, Qunfang.,...&Jiang, Guibin.(2019).Butylated hydroxyanisole isomers induce distinct adipogenesis in 3T3-L1 cells.JOURNAL OF HAZARDOUS MATERIALS,379,-.
MLA	Sun, Zhendong,et al."Butylated hydroxyanisole isomers induce distinct adipogenesis in 3T3-L1 cells".JOURNAL OF HAZARDOUS MATERIALS 379(2019):-.