2,3,7,8-Tetrachloodibenzo-p-dioxin affects the differentiation of CD4 helper T cell

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	2,3,7,8-Tetrachloodibenzo-p-dioxin affects the differentiation of CD4 helper T cell
	Pang, Chengfang 7; Zhu, Conghui 6; Zhang, Yuanyuan 5; Ge, Ying 3,4; Li, Shujuan 6; Huo, Shouliang 1; Xu, Tuan ; Stauber, Roland H.; Zhao, Bin 9
刊名	TOXICOLOGY LETTERS
	2019-09-01
卷号	311 页码:49-57
关键词	Dioxin TCDD Helper T cell Cell differentiation Immune suppression AhR
ISSN号	0378-4274
英文摘要	2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener of dioxins, is a persistent and ubiquitous environmental contaminant. Although the immunotoxic effects of TCDD have been reported, the mechanisms underlying these effects are still unclear. In this study, we have determined the toxic effects of TCDD on thymocytes and splenic T cells with in vitro cell culture systems. Magnetically isolated mouse splenic Th cells, Treg cells and the mixed spleen lymphocytes (SLC) were cultured and treated with TCDD and the differentiation of CD4 Th cells was determined by flow cytometery. Our results showed that different concentrations of TCDD caused immunotoxic effects through different toxicological mechanisms in both the purified mouse splenic Th cells and the mixed SLC. The low dose exposure to TCDD triggered regulatory effects in the immune system, while the high dose TCDD exposure resulted in severe immune toxicity. Notably, a decline of Treg subset was observed, suggesting an imbalanced immune regulation by TCDD treatment, as well as a possible decrease of TCDD's indirect effects on bystander immune cells. Our CD4 Th subset co-culture experiments showed that TCDD-induced pathobiology depended on immune cell balance, suggesting that cytokine-induced micro-environments further modulated toxic effects associated with TCDD exposure.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42489]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Univ Copenhagen, Biotech Res & Innovat Ctr, DK-2200 Copenhagen, Denmark 2.Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China 3.Univ Copenhagen, Rigshosp, Fac Hlth & Med Sci, Finsen Lab, DK-2200 Copenhagen, Denmark 4.Linyi Peoples Hosp, Dept Endocrinol, Linyi 276003, Shandong, Peoples R China 5.Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China 6.Tech Univ Denmark, Dept Environm Engn, DK-2800 Lyngby, Denmark 7.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Shuangqing Rd 18, Beijing 100085, Peoples R China 8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 9.Stauber, Roland H.] Univ Med Ctr Mainz, Dept Nanobiomed ENT, Langenbeckstr 1, D-55101 Mainz, Germany
推荐引用方式 GB/T 7714	Pang, Chengfang,Zhu, Conghui,Zhang, Yuanyuan,et al. 2,3,7,8-Tetrachloodibenzo-p-dioxin affects the differentiation of CD4 helper T cell[J]. TOXICOLOGY LETTERS,2019,311:49-57.
APA	Pang, Chengfang.,Zhu, Conghui.,Zhang, Yuanyuan.,Ge, Ying.,Li, Shujuan.,...&Zhao, Bin.(2019).2,3,7,8-Tetrachloodibenzo-p-dioxin affects the differentiation of CD4 helper T cell.TOXICOLOGY LETTERS,311,49-57.
MLA	Pang, Chengfang,et al."2,3,7,8-Tetrachloodibenzo-p-dioxin affects the differentiation of CD4 helper T cell".TOXICOLOGY LETTERS 311(2019):49-57.