Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine

doi:10.1002/smtd.202000491

	Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine
	Padhiar, Arshad Ahmed 8,9; Faqeer, Abdullah 10; Sun, Shimin 1; Hamid, Md Rana 8,9; Liao, Jinqi 8,9; Yan Zhou 2; Ahmmed, Bulbul 8,9; Qin, Xialing 10; Tie Changjun 3; Gao, Shuping 4
刊名	SMALL METHODS
	2020-10-08
页码	13
关键词	hepatic transporters magnetic resonance imaging mesenchymal stem cells near-infrared region non-invasive multimodality imaging
ISSN号	2366-9608
DOI	10.1002/smtd.202000491
通讯作者	Chen Zhang(czhang@ccmu.edu.cn) ; Zhou, Guangqian(gqzhou@szu.edu.cn)
英文摘要	Hepatic transporters facilitate the uptake of xenobiotic compounds and confer immense potential as multimodality reporters, as they can uptake multitude of bioluminescent, fluorescence, near Infra-red (NIR), and magnetic resonance imaging (MRI) compounds. Here, it is aimed to identify the prospects of three human hepatocyte membrane proteins, organic anion transporters (OATP) 1B1, OATP1B3, and sodium-taurocholate cotransporting polypeptide in tracing the transplanted cells dynamics, by the uptake of clinically approved MRI compound, gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (GD-EOB DTPA) or NIR fluorescent dye, Indocyanine Green (ICG). First, OATP1B1 is introduced as new MRI reporter which is the human orthologue of previously established rodent (Oatp1a1) reporter. Comparative sequential assays by assimilating MR image parameters reveal OATP1B3 as superior reporter on T1-weighted images and display highest contrast enhancement reported to-date when using a clinical 3T MR scanner (approximate to 21-fold in vitro; approximate to 8-fold in vivo). Stably expressing these hepatic transporters have no effect on human adipose derived mesenchymal stem cells (hADSCs) characteristics. However, only OATP1B3 able to trace as few as 2 x 10(5)hADSCs intramuscular xenograft survival time on NIR and 3T MRI. These data suggest that OATP1B3 is relatively a robust Gd-EOB-DTPA/ICG-dependent multimodality reporter in visualizing dynamic processes for cell-based therapies.
资助项目	National Key R&D Program of China[2017FA105202] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170818094109846] ; Shenzhen Science and Technology Innovation Committee[GJHZ20180928155604617] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170307155449972] ; Natural Science Foundation[81772333] ; Natural Science Foundation[81774053]
WOS关键词	INDOCYANINE GREEN ; EXPRESSION ; FLUORESCENCE
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000575956200001
资助机构	National Key R&D Program of China ; Shenzhen Science and Technology Innovation Committee ; Natural Science Foundation
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/42068]
专题	自动化研究所_脑网络组研究中心
通讯作者	Chen Zhang; Zhou, Guangqian
作者单位	1.Shenzhen Univ, Hlth Sci Ctr, Guangdong Key Lab Genome Stabil & Human Dis Preve, Shenzhen, Peoples R China 2.Lungene Technol, 8606,Yinxing Sci Bldg, Shenzhen 510086, Peoples R China 3.Chinese Acad Sci, Paul C Lauterbur Res Ctr Biomed Imaging, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China 4.Peking Univ, Sch Life Sci, PKU IDG McGovern Inst Brain Res, State Key Lab Membrane Biol, Beijing 100871, Peoples R China 5.Guangzhou Univ Chinese Med, Shenzhen Tradit Chinese Med Hosp, Dept Pathol, Clin Med Coll 4, Guangzhou 518033, Peoples R China 6.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Inst Automat, Beijing 100190, Peoples R China 7.Chinese Acad Sci, Natl Lab Pattern Recognit, Beijing 100190, Peoples R China 8.Shenzhen Univ, Hlth Sci Ctr, Shenzhen Key Lab Antiaging & Regenerat Med, Dept Med Cell Biol & Genet,Guangdong Key Lab Geno, Shenzhen 518060, Peoples R China 9.Shenzhen Univ, Hlth Sci Ctr, Shenzhen Engn Lab Regenerat Technol Orthopaed Dis, Shenzhen 518060, Peoples R China 10.Shenzhen Univ, Sch Biomed Engn, Hlth Sci Ctr, Shenzhen 518060, Peoples R China
推荐引用方式 GB/T 7714	Padhiar, Arshad Ahmed,Faqeer, Abdullah,Sun, Shimin,et al. Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine[J]. SMALL METHODS,2020:13.
APA	Padhiar, Arshad Ahmed.,Faqeer, Abdullah.,Sun, Shimin.,Hamid, Md Rana.,Liao, Jinqi.,...&Zhou, Guangqian.(2020).Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine.SMALL METHODS,13.
MLA	Padhiar, Arshad Ahmed,et al."Utility of Hepatic Transporters as Multimodal Gene Reporters for Cell-Based Medicine".SMALL METHODS (2020):13.