Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics | |
Wang Sheng-Xiong6,7; Chen Zhen-Qiang5; Han Hua4; Guo Ai-Ke1,2,3,6,7 | |
刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS |
2020-10-01 | |
卷号 | 47期号:10页码:1090-1096 |
关键词 | brain connectomics volume electron microscopy sample preparation |
ISSN号 | 1000-3282 |
DOI | 10.16476/j.pibb.2020.0011 |
通讯作者 | Han Hua() ; Guo Ai-Ke(akguo@ion.ac.cn) |
英文摘要 | For micro-brain connectomic research, volume electron microscopy is a main method in which it plays an importatnt role for the appliaction of automated tape-collecting ultramicrotome (ATUM) system and arrayimaging with scanning electron microscopy together. ATUM system enables collecting a large amount of special sections to be imaged with SEM to form ahuge dataset. With regard tothe nature of non-vacuum sectoningand sample reserving. we modified and improved the sample preparatio process includingresinrecipe, HRP labelling and post-staing method i orderto reduce folding rate of sample sections and make it possible for identifyig certain types of neurons in high-contrast imaging dataset, thus obtainig dataset of high quality for connectomic research. |
资助项目 | Strategic Priority Research Program of Chinese Academy of Science[XDB32010100] |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
语种 | 英语 |
出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
WOS记录号 | WOS:000583458700007 |
资助机构 | Strategic Priority Research Program of Chinese Academy of Science |
内容类型 | 期刊论文 |
源URL | [http://ir.ia.ac.cn/handle/173211/41748] |
专题 | 类脑智能研究中心_微观重建与智能分析 |
通讯作者 | Han Hua; Guo Ai-Ke |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China 2.Shanghai Ctr Brain Sci & Inspired Intelligence Te, Shanghai 201210, Peoples R China 3.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China 4.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Luwan Branch, Shanghai 200020, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, State Key Lab Brain & Cognit Sci, Inst Biophys, Beijing 100101, Peoples R China |
推荐引用方式 GB/T 7714 | Wang Sheng-Xiong,Chen Zhen-Qiang,Han Hua,et al. Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2020,47(10):1090-1096. |
APA | Wang Sheng-Xiong,Chen Zhen-Qiang,Han Hua,&Guo Ai-Ke.(2020).Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,47(10),1090-1096. |
MLA | Wang Sheng-Xiong,et al."Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 47.10(2020):1090-1096. |
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