Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform | |
Song, Fengge1; Wei, Yangdao1; Wang, Peng4; Ge, Xiaolin1; Li, Chaoyang1; Wang, Aimin1; Yang, Zhiqing1; Wan, Yi1,3,4; Li, Jinghong2 | |
刊名 | BIOSENSORS & BIOELECTRONICS |
2021-08-01 | |
卷号 | 185页码:5 |
关键词 | Bacterial diagnostics CRISPR-Cas14a Tag-specific primer extension Magneto-DNA |
ISSN号 | 0956-5663 |
DOI | 10.1016/j.bios.2021.113262 |
通讯作者 | Yang, Zhiqing(yangzhiqing@hainanu.edu.cn) ; Wan, Yi(993602@hainu.edu.cn) |
英文摘要 | Nucleic acid-based diagnosis using CRISPR-Cas associated enzymes is essential for rapid infectious disease diagnosis and treatment strategies during a global pandemic. The obstacle has been blossomed CRIPSR-Cas based tools that can monitor wide range of pathogens in clinical samples with ultralow concentrations. Here, a universal nucleic acid magneto-DNA nanoparticle system was exploited for the detection of pathogenic bacteria, based on the collateral cleavage activity of CRISPR-Cas14a and tag-specific primer extension. In the system, the target nucleic acids were amplificated and be separated from mixtures by streptavidin-coated magnetic bead. The collateral cleavage activity of CRISPR-Cas14a can be activated via the tag sequence on the target product. Consequently, the fluorophore quencher reporter can be activated by CRISPR-Cas14a, leading to the increasing response. The exploited universal bacterial diagnostic can distinguish six different bacteria strains with 1 cfu/mL or 1 aM sensitivity, which may provide new strategies to construct fast, accurate, cost-effective and sensitive diagnostic tools in environments with limited resources. |
资助项目 | Hainan Provincial Natural Science Foundation of China[420RC518] ; Open Fund of Shandong Key Laboratory of Corrosion Science[KLCS201910] ; Tsinghua University Initiative Scientific Research Program[2020Z99CFZ019] ; Tsinghua University Spring Breeze Fund[2020Z99CFZ019] ; Research Foundation of Hainan University[KYQD (ZR) 1711] ; Research Foundation of Hainan University[KYQD (ZR) 1997] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF04] ; Open fund of Fujian Provincial Key Laboratory of Photonics Technology[JYG 2002] |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
语种 | 英语 |
出版者 | ELSEVIER ADVANCED TECHNOLOGY |
WOS记录号 | WOS:000655570200001 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/171001] |
专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
通讯作者 | Yang, Zhiqing; Wan, Yi |
作者单位 | 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Bio Fouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Song, Fengge,Wei, Yangdao,Wang, Peng,et al. Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform[J]. BIOSENSORS & BIOELECTRONICS,2021,185:5. |
APA | Song, Fengge.,Wei, Yangdao.,Wang, Peng.,Ge, Xiaolin.,Li, Chaoyang.,...&Li, Jinghong.(2021).Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform.BIOSENSORS & BIOELECTRONICS,185,5. |
MLA | Song, Fengge,et al."Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform".BIOSENSORS & BIOELECTRONICS 185(2021):5. |
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